INTROGRESSION BREEDING IN GENUS TULIPA ANALYSED BY GISH

Interspecific hybridization is an important tool in tulip breeding to transfer some important horticultural traits into new cultivars.
The diploid (2n=2x=24) F₁ interspecific hybrids between T. gesneriana × T. fosteriana (GF) were backcrossed to diploid T. gesneriana cultivar ‘Yellow Flight’ (G). BC₁ plants resulting from these crosses were analysed by genomic in situ hybridization (GISH) to estimate their genome constitution and extent and position of intergenomic recombination.
All of the BC₁ progenies analysed were diploid (2n=2x=24). GISH enabled unequivocally the identification of parental chromosomes as well as the recombinant chromosomes.
Because the T. gesneriana parent was used for backcrossing, the number of G genome chromosomes (chromosomes with centormere of T. gesneriana genome) predominated in the BC₁ progenies.
The total number of T. fosteriana chromosomes in hybrids ranged from four to ten.
There were two types of recombinant chromosomes.
Those with a centromere of T. fosteriana chromosome with recombinant segment of T. gesneriana (F/G) and vice versa (G/F). Most recombinant chromosomes contained a single crossover.
The percentages of T. fosteriana chromatin in BC₁ hybrids ranged from 20 to 24%.

XXIII International Eucarpia Symposium, Section Ornamentals: Colourful Breeding and Genetics

A. Marasek-Ciolakowska, M.S. Ramanna, J.M. van Tuyl

Tulipa, backcross progenies, chromosome recombination, genome analysis, interspecific hybridization, karyotyping

https://doi.org/10.17660/ActaHortic.2009.836.14