Articles
EARLY FLOWERING TRANSGENIC CHRYSANTHEMUM PLANTS
Article number
836_34
Pages
241 – 246
Language
English
Abstract
Chrysanthemum (Chrysanthemum morifolium) is one of the worldwide ornamental plants.
Early-flowering cultivars are favourable to produce quality chrysanthemum cut flowers at lower cost of production.
Flowering control should allow shorter breeding cycles.
A possible strategy to speed up flowering in plants is genetic manipulation of key regulators of floral transition.
For example, constitutive expression of LFY and AP1 genes cause early flowering of transgenic plants.
We cloned three AP1-homologous genes from chrysanthemum (CDM111) and sunflower (HAM75, HAM92) and studied chrysanthemum transgenic plants that overexpressed these genes.
In total 37 independently regenerated plants carrying integrated transgenes were produced and used for subsequent experiments on flowering induction.
We show that over-expression of compositae AP1-homologs in transgenic chrysanthemum under long-day conditions has no effect on plant development.
However, under short-day conditions transgenic plants have started bud initiation two weeks earlier than nontransgenic control chrysanthemum plants.
Later on, transgenic chrysanthemum plants colored earlier and an earlier harvesting by 5 weeks was possible compared to nontransgenic control plants.
These results open new possibilities for genetic improvement and breeding of chrysanthemum cultivars.
Early-flowering cultivars are favourable to produce quality chrysanthemum cut flowers at lower cost of production.
Flowering control should allow shorter breeding cycles.
A possible strategy to speed up flowering in plants is genetic manipulation of key regulators of floral transition.
For example, constitutive expression of LFY and AP1 genes cause early flowering of transgenic plants.
We cloned three AP1-homologous genes from chrysanthemum (CDM111) and sunflower (HAM75, HAM92) and studied chrysanthemum transgenic plants that overexpressed these genes.
In total 37 independently regenerated plants carrying integrated transgenes were produced and used for subsequent experiments on flowering induction.
We show that over-expression of compositae AP1-homologs in transgenic chrysanthemum under long-day conditions has no effect on plant development.
However, under short-day conditions transgenic plants have started bud initiation two weeks earlier than nontransgenic control chrysanthemum plants.
Later on, transgenic chrysanthemum plants colored earlier and an earlier harvesting by 5 weeks was possible compared to nontransgenic control plants.
These results open new possibilities for genetic improvement and breeding of chrysanthemum cultivars.
Publication
Authors
O.A. Shulga, T.YU. Mitiouchkina, A.V. Shchennikova, K.G. Skryabin, S.V. Dolgov
Keywords
Chrysanthemum morifolium (Ramat.), MADS-box transcription factors, genetic transformation
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