Articles
IN VITRO MUTAGENESIS IN ASTER NOVI-BELGII CULTIVARS
Article number
836_29
Pages
207 – 214
Language
English
Abstract
An in vitro culture system based on adventitious shoot formation was developed for Aster novi-belgii and used for mutagenic treatment.
From leaf blade sections adventitious shoots were produced within eight weeks on a Murashige and Skoog based medium with 1 mg/L benzyladenine.
For mutagenesis in vitro shoots were irradiated with gamma rays prior to adventitious shoot formation.
Radiation above 20 Gy was detrimental to adventitious shoot formation.
However, the percentage of putative mutants seemed to be only slightly affected by radiation dose.
Plants regenerated after exposure to gamma radiation were grown in a greenhouse and evaluated with special interest on flower characteristics, i.e. colour, size and number of whorls of ray florets. Victoria Fanny produced more mutants and more interesting mutants than Victoria Jane. Flowers with different intensities of pink, red-purple and dark-purple colours were recorded.
Ray floret whorls varied in number from a single whorl to flowers with only ray florets in the capitula (and missing disc florets). Differences in curling and twisting of ray florets, as well as variation in plant height, branching ability and time to flowering were present among the regenerants.
Despite regeneration via adventitious shoots plants showing chimerism were found among the mutants.
In vitro mutagenesis of Aster novi-belgii is a promising breeding tool; however, the potential of this tool depends on the cultivar used for mutagenic treatment and chimerism cannot be avoided.
From leaf blade sections adventitious shoots were produced within eight weeks on a Murashige and Skoog based medium with 1 mg/L benzyladenine.
For mutagenesis in vitro shoots were irradiated with gamma rays prior to adventitious shoot formation.
Radiation above 20 Gy was detrimental to adventitious shoot formation.
However, the percentage of putative mutants seemed to be only slightly affected by radiation dose.
Plants regenerated after exposure to gamma radiation were grown in a greenhouse and evaluated with special interest on flower characteristics, i.e. colour, size and number of whorls of ray florets. Victoria Fanny produced more mutants and more interesting mutants than Victoria Jane. Flowers with different intensities of pink, red-purple and dark-purple colours were recorded.
Ray floret whorls varied in number from a single whorl to flowers with only ray florets in the capitula (and missing disc florets). Differences in curling and twisting of ray florets, as well as variation in plant height, branching ability and time to flowering were present among the regenerants.
Despite regeneration via adventitious shoots plants showing chimerism were found among the mutants.
In vitro mutagenesis of Aster novi-belgii is a promising breeding tool; however, the potential of this tool depends on the cultivar used for mutagenic treatment and chimerism cannot be avoided.
Publication
Authors
K. Kristiansen, K.K. Petersen
Keywords
adventitious shoots, chimerism, gamma radiation, mutant
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