Articles
CONVERSION OF MOLECULAR MARKERS LINKED TO FUSARIUM AND VIRUS RESISTANCE IN ASIATIC LILY HYBRIDS
Article number
836_18
Pages
131 – 136
Language
English
Abstract
Lilies are one of the most economically important monocot flower bulbs.
However, lily bulb production faces some challenges such as susceptibility to diseases caused by Fusarium oxysporum and Lily Mottle Virus (LMoV). These two are the most important pathogens that cause serious damage.
Fortunately, some Asiatic lily hybrids show high level of resistance to Fusarium and LMoV. Transmission of these traits into the lily assortment through introgression is possible however combining several loci by conventional breeding will take a long time.
Using marker-assisted selection (MAS) could speed up the breeding process considerably.
A genetic map of an intraspecific Asiatic backcross population was constructed using three different molecular marker systems (DArT, AFLP and NBS profiling) and four QTLs for Fusarium oxysporum and one locus for LMoV were localized on this map.
The most tightly linked markers to the most significant two Fusarium QTLs in the AA population were converted into more robust PCR markers.
Thus, the first markers for MAS breeding in the very huge and highly repetitive genome of lily have been developed.
However, lily bulb production faces some challenges such as susceptibility to diseases caused by Fusarium oxysporum and Lily Mottle Virus (LMoV). These two are the most important pathogens that cause serious damage.
Fortunately, some Asiatic lily hybrids show high level of resistance to Fusarium and LMoV. Transmission of these traits into the lily assortment through introgression is possible however combining several loci by conventional breeding will take a long time.
Using marker-assisted selection (MAS) could speed up the breeding process considerably.
A genetic map of an intraspecific Asiatic backcross population was constructed using three different molecular marker systems (DArT, AFLP and NBS profiling) and four QTLs for Fusarium oxysporum and one locus for LMoV were localized on this map.
The most tightly linked markers to the most significant two Fusarium QTLs in the AA population were converted into more robust PCR markers.
Thus, the first markers for MAS breeding in the very huge and highly repetitive genome of lily have been developed.
Publication
Authors
A. Shahin, P. Arens, S. van Heusden, J.M. van Tuyl
Keywords
Lily mottle virus, Fusarium oxysporum, genetic map, DArT, NBS profiling, MAS
Online Articles (40)