Articles
DEVELOPMENT OF COLORED, NON-VERNALIZATION-REQUIRING SEED PROPAGATED LILIES
Article number
836_27
Pages
193 – 198
Language
English
Abstract
Recent discovery of seed-propagated Lilium × formolongi hybrids that flower continuously in <1 yr without vernalization (200-240d after sowing due to ≥1 dominant VER1, VER2 alleles) with frost-tolerance, day neutrality, and winter hardiness is an unprecedented combination in Lilium. Our objective to obtain colored L. × formolongi was to use Class I lilies that initiate flower buds prior to a cold treatment as parents, e.g. Lilium martagon. Reciprocal interspecific crosses between fertile parents (1-L. × formolongi, 7-colored L. martagon) were made in both directions to generate segregating hybrid (F1) and inbred (F2) progenies.
A total of 8,826 F1 seeds or embryos were generated.
Embryo rescue was employed when L. martagon was the female, although viable embryos never germinated after ~1 yr in culture.
Mean germination ranged from 0-1.02% for in situ ripened seed.
Hybridity for one successful cross (07L-14; L. × formolongi [00L-111-343×51-202-1] × L. martagon Cadense) was analyzed using morphological (flowering, leaf and internode number, leaf length:width ratios, compatibility, no. flower stalks and flowers, rosetting) and molecular markers (ISSR primers). Twenty-two 07L-14 genotypes were genetically similar to the female parent whereas 86 aligned closely with the male (Cadense). The number of shoots/plant was the only quantitative trait co-segregating with ISSRs.
Flowering traits were intermediate to, but significantly different than, either parent.
A majority of the plant height and inflorescence lengths were significantly greater than the parents.
Two F1s had slight flower coloration in the petals and tepals; one hybrid had an open-faced rather than trumpet flower (similar to an Oriental type).
A total of 8,826 F1 seeds or embryos were generated.
Embryo rescue was employed when L. martagon was the female, although viable embryos never germinated after ~1 yr in culture.
Mean germination ranged from 0-1.02% for in situ ripened seed.
Hybridity for one successful cross (07L-14; L. × formolongi [00L-111-343×51-202-1] × L. martagon Cadense) was analyzed using morphological (flowering, leaf and internode number, leaf length:width ratios, compatibility, no. flower stalks and flowers, rosetting) and molecular markers (ISSR primers). Twenty-two 07L-14 genotypes were genetically similar to the female parent whereas 86 aligned closely with the male (Cadense). The number of shoots/plant was the only quantitative trait co-segregating with ISSRs.
Flowering traits were intermediate to, but significantly different than, either parent.
A majority of the plant height and inflorescence lengths were significantly greater than the parents.
Two F1s had slight flower coloration in the petals and tepals; one hybrid had an open-faced rather than trumpet flower (similar to an Oriental type).
Publication
Authors
N.O. Anderson, A. Younis, E. Optiz
Keywords
interspecific hybridization, hybrid breakdown, VER1, VER2, self incompatibility, self compatibility, ISSRs
Online Articles (40)