Articles

DEVELOPMENT OF REALTIME ASSAY FOR THE DETECTION OF STRAWBERRY CRINKLE VIRUS

Article number
842_34
Pages
223 – 226
Language
English
Abstract
Viruses represent one of the major threats for the strawberry industry, causing important economic losses, and their control is based mainly on rigorous certification programs to maintain clean planting stocks; these viruses include SCV (Strawberry Crinkle Virus) the causal agent of a quarantine disease of major economic importance.
All strawberry cultivars are susceptible to SCV infection but due to the absence of obvious symptoms, the detection is limited to indexing on strawberry indicator plants, an expensive and time-consuming procedure.
The aim of this study is to develop a sensitive, reliable and fast RealTime assay, based on TaqMan chemistry and amplifying a portion of the conserved L gene sequence coding for RNA-dependent RNA polymerase (RdRp). As strawberry is a difficult host for virus detection, an evaluation of the efficiency of 4 different RNA extraction methods and a comparison between conventional nested-PCR and RealTime assay were carried out.
RealTime detection of SCV in several symptomatic and asymptomatic strawberry plants, field collected, indexed on strawberry indicator plants and/or maintained under CAV’s screen-houses, proved highly efficient and reproducible, and its sensitivity was as high as the nested PCR assay.
This new protocol represents an improvement on the existing analytical methods and could be used as a reliable diagnostic procedure for routine tests in certification and control programs.

Publication
Authors
S. Botti, M. Cardoni
Keywords
diagnosis, SCV, Certification Program, TaqMan assay, n-RT-PCR
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P.A. Dávalos-González | J. Narro-Sánchez | A.E. Jofre-Garfias | A.R. Hernández Razo | M.N. Vázquez-Sánchez
P. Tongtraibhop | T. Thongthieng | C. Nuengchaknin | C. Pitakpittaya
T. Mochizuki | Y. Yoshida | T. Yanagi | M. Okimura | A. Yamasaki | H. Takahashi
L.M.F. Castañeda | L.E.C. Antunes | N.C. Ristow | S. Carpenedo
Jong-Bun Seo | Gil-Ho Shin | Kyung-Chul Cho | Joung-Keun Kim | Kyong-Ju Choi | Won-Mo Yang
A. Domínguez | E. Martínez | A. Trigo | D. Alonso | R. García | R. Sánchez | R. Ghorbel | J. Tomás
G. Caracciolo | A. Moncada | C. Prinzivalli | F. D'Anna
C. Reisser Júnior | J.C. Carvalho | L.M.F. Castañeda | N.C. Ristow | S. Carpenedo | L.E. Corrêa Antunes
S.K. Kim | H.Y. Na | J.H. Song | M.J. Kim | J.E. Son | R.N. Bae | C. Chun | H.J. Kang
Zhihong Zhang | Linlin Chang | Hongyi Yang | Min Xiao | He Li | Hongyan Dai
O. Daugovish | M. Mochizuki | S. Fennimore
C. Redondo | A. De Cal | A. Martínez-Treceño | M. Becerril | J.M. López-Aranda | P. Melgarejo
C. Redondo | A. De Cal | A. Martínez-Treceño | E. García | D. García-Sinovas | M. Becerril | J.M. López-Aranda | P. Melgarejo
K. Olbricht | G. Staudt | P. Becker | I. Szankowski
F.T. Arroyo | B. De los Santos | F. Romero | J. Moreno | P. Daza | J. Torreblanca
L. Antoniacci | E. De Paoli | C. Montuschi | G. Ceredi | S. Gengotti
B. De los Santos | C. Blanco | F.T. Arroyo | Y. Llergo | F. Romero | C. Soria | J.F. Sánchez-Sevilla | J.M. López-Aranda
J. Cubero | M.A. Ayllón | I. Gell | P. Melgarejo | A. De Cal | P.M. Martín-Sánchez | R.M. Pérez-Jiménez | C. Soria | E. Segundo | I. Larena
A. Aguado | B. De los Santos | F.T. Arroyo | Y. Llergo | C. Blanco | J. Ortega | F. Romero
A. Sombardier | L. Willocquet | D. Blancard | M.F. Corio-Costet | S. Savary | A. Bardet | C. Eckert | Y. Trottin-Caudal | B. Denoyes-Rothan | J.J. Pommier | A. Petit | P. Chartier
N.C. Ristow | S. Carpenedo | R. Trevisan | L.E.C. Antunes | C.J. da Silva Freire
M. Avilés | S. Castillo | C. Borrero | M.L. Castillo | T. Zea-Bonilla | R.M. Pérez-Jiménez
M. Abril | K.J. Curry | A.J. DeLucca | S. Boue | B.J. Smith | D.E. Wedge
M. Mari | S. Gengotti | G. Ceredi | L. Antoniacci | C. Montuschi | E. De Paoli
V.C. Conci | A.K. Torrico | E. Cafrune | V. Quevedo | O. Baino | J.C. Ramallo | A.M. Borquez | V.A. Mollinedo | J.J. Agüero | D.S. Kirschbaum
M. Ragab | K. El-Dougdoug | S. Mousa | A. Attia | I. Sobolev | S. Spiegel | S. Freeman | M. Zeidan | I.E. Tzanetakis | R.R. Martin
R.M. Pérez-Jiménez | E. Segundo | M.P. Carmona | A. González-Vizcaíno | J. Gálvez Farfán | T. Zea-Bonilla | P.M. Martín-Sánchez | I. Amaya | C. Soria | J.M. López-Aranda | J.F. Sánchez-Sevilla
G. Ceredi | L. Antoniacci | C. Montuschi | E. De Paoli | M. Mari | S. Gengotti
T. Kanto | K. Matsuura | M. Yamada | T. Usami | Y. Amemiya
J. Soliveri | M. Arenas | J.L. Copa-Patiño | J.L. Caballero | J. Muñoz-Blanco
P. Parikka | P. Kivijärvi | S. Prokkola | R. Kemppainen
M. Gaskell | M.P. Bolda | J. Muramoto | O. Daugovish
J.C. Shih | T.F. Hsieh | C.C. Ho
Qing-Hua Gao | Zheng-wen Ye | Hong-qing Zheng | Xue-ying Zhang
Li Yang | Li Li | Lei Yang | Baochun Hao | Fengjie Chu
E. Vicente | G. Giménez | A. Manzzioni | F. Vilaró | M. González | M. Cabot
T. Yanagi | P. Nathewet | N. Okuda | K. Sone
P.A. Dávalos-González | J. Narro-Sánchez | A.E. Jofre-Garfias | A.R. Hernández Razo | M.N. Vázquez-Sánchez
A. Refoyo | J.M. Arenas | S. Castillo | M. Avilés
K. Sone | M. Okimura | E. Kitadani | T. Mochizuki | Y. Noguchi | H. Yoshida
E. Montironi | E. Costantini | B. Mezzetti | F. Mourgues | C. Rosati
F. Capocasa | J. Diamanti | S. Pierini | B. Mezzetti
H. Takahashi | A. Yamasaki | K. Shoji | K. Kawagishi | T. Taguchi | Y. Yoshida | M. Morishita
G. Staudt | S. Schneider | P. Scheewe | D. Ulrich | K. Olbricht
M.E. Herrington | L. Woolcock | M. Wegener | M. Dieters | J. Moisander
C. Soria | J.J. Medina | J.F. Sánchez-Sevilla | R. Bartual | A. Refoyo | J. Gálvez | L. Miranda | R. Villalba | M.T. Ariza | J.M. López-Aranda
M.D. Vitten | K. Olbricht | F. Tiedke
M. Alsheikh | R. Sween | A. Nes | M. Gullord
N.C. Ristow | S. Carpenedo | C. Reisser Junior | A.C. Richter Krolow | J.E. Schwengber | L.E.C. Antunes
D.W. Simpson | K.J. Hammond | A.W. Johnson | A.J. Passey | A.B. Whitehouse
T.M. Davis | M.E. Shields | Qian Zhang | E.G. Poulsen | J.L. Bennetzen | K.M. Folta | P. San Miguel
B. Denoyes-Rothan | M. Rousseau-Gueutin | A. Gaston | D. Roca | N. Pedeprat | E. Lerceteau-Köhler | L. Barrot | A. Petit | P. Chartier
A. Moncada | G. Caracciolo | C. Prinzivalli | F. D'Anna
W. Faedi | L. Ballini | G. Baroni | G. Baruzzi | M. Baudino | R. Giordano | P. Lucchi | M.L. Maltoni | M. Migani | L. Placchi
W. Faedi | G. Baruzzi | G. Capriolo | F. D'Anna | P. Lucchi | A. Marano | G. Martelli | C. Mennone | C. Prinzivalli
G. Baruzzi | W. Faedi | P. Lucchi | M. Migani | P. Sbrighi | P. Turci
J.A. García-Gago | J.M. López-Aranda | J. Muñoz-Blanco | F.J. Toro | M.A. Quesada | F. Pliego-Alfaro | J.A. Mercado
W. Njuguna | N.V. Bassil | K.E. Hummer | C. Richards | T.M. Davis
Zhao Mizhen | Wu Weimin | Lei Jiajun | Qian Yaming | Wang Zhuangwei | Yuan Ji
Wu Weimin | Zhao Mizhen | Wang Zhuangwei | Qian Yaming | Yuan Ji
M. Nikolić | J. Milivojević | M. Ivanović
Shuping Yin | K.D. Larson
Jeng-Hsien Tsai | Gwang-Miao Chang | Tian-Yih Wu | Shu-Chen Peng | Hsun-Yao Chang | Su-Jein Chang
B. Mezzetti | R. Nestby | M. Korbin | B. Denoyes-Rothan | B. van Duijn | M. Bodson
M. Turquet | J.J. Pommier | M. Piron | E. Lascaux | G. Lorin
T. Tuovinen | I. Lindqvist | S. Kauppinen | P. Kivijärvi
T. Ikeda | K. Yamazaki | H. Kumakura | H. Hamamoto
D. García-Sinovas | E. García-Méndez | M.A. Andrade | A. de Cal | P. Melgarejo | T. Salto | M.L. Martínez-Beringola | C. Redondo | A. Martínez-Treceño | M. Becerril | J.J. Medina | C. Soria | J.M. López-Aranda
E. García-Méndez | D. García-Sinovas | M.A. Andrade | B. González | J.F. Sánchez-Sevilla | J.M. López-Aranda | J.J. Medina | L. Miranda
A. Martínez-Treceño | P. Chomé | M. Becerril | C. Soria | J.J. Medina | J.M. López-Aranda
F. D'Anna | A. Moncada | G. Caracciolo | L. Guarino | A. Virzì | P. Spata
E.Z. Brandán | R.R. Fernández | E.L. Villagra | S.M. Salazar | C. Cruz | R. Ponce de León
M.S.S. El-Shabasi | M.E. Ragab | I.I. El-Oksh | Y.M.M. Osman
L. Landi | E. Costantini | E. Montironi | B. Mezzetti
M.T. Ariza | J.M. López-Aranda | M.T. Cid-Atencia | C. Soria | J.J. Medina | L. Miranda
A.W. Johnson | O.M. Grant | M.J. Davies | C.M. James | A.J. Passey | D.W. Simpson
J. Rohloff | A.M. Bones | P. Winge | T.S. Jørstad | J. Kopka | A. Erban | A.M. Bones | T. Sparstad | R.C. Wilson | S.K. Randall | J. Davik | M. Alsheikh
V. Luko¿evičiūtė | R. Rugienius | I. Zalunskaite | A. Sasnauskas | V. Stanys
Y.T. Zhang | J. Kong | T.Z. Li | Z.H. Han | G.X. Wang | J. Dong | C.F. Zhong
G. Sarli | A. De Lisi | V. Montesano | D. Schiavone
A. Bolognesi | G. Baruzzi | A. Chatgilialoglu | M. Bortolotti | L. Polito | W. Faedi
R.F.F. Cantillano | L.E.C. Antunes | L.M. Castañeda | R.O. Treptow
N. Tsukamoto | K. Fukuhara | X.X. Li | S. Yamashita | S. Yui | Y. Inaba | M. Okimura | H. Ikeura | Y. Hayata
H. Ikeura | N. Tsukamoto | K. Fukuhara | X.X. Li | S. Yamashita | S. Yui | Y. Inaba | M. Okimura | M. Tamaki | Y. Hayata
J.M. Silva | L.C.S.A. Correia | N.P. Moura | P.L. Salgado | M.I.S. Maciel | H.P. Villar
M.B. Sousa | T. Curado | M.J. Trigo | F.N. Vasconcelos | T. Nunes
C.R. Figueroa | R. Herrera | P. Pimentel | P.D.S. Caligari | M.A. Moya-León
X.X. Li | N. Tsukamoto | K. Fukuhara | S. Yamashita | T. Sakamoto | M. Okimura | Y. Hayata
J.G. Pádua | J. Duarte Filho | C.M. Caproni | R.V. da Mota | L.E.C. Antunes | E.L. do Carmo
E. Kafkas | N. Kemal Koc | S. Paydas Kargı | M.A. Unlü | K. Erçik | A. Kiminsu
M.A. Quesada | S. Posé | N. Santiago-Doménech | R. Sesmero | M.C. Molina | S. Mousa | F. Pliego-Alfaro | J.A. Mercado | J.L. Caballero | J. Muñoz-Blanco | L. Trainotti | G. Casadoro | J.A. García-Gago | J.M. López-Aranda
A.M. León | P. Torres | C. Sanz | A.G. Pérez
S. Voća | B. Duralija | J. Dru¿ić | N. Dobričević | V. Dragović-Uzelac
I. Williams | J.C. Williams | L. Hernández | G. Maya
M.G. Palha | J.L. Campo | L. Reis | T. Curado | M.B. Sousa | C.S. Andrade
J.J. Heller | PH. Sunder | P. Charles | J.J. Pommier | J. Fritsch
J.M. López-Aranda | L. Miranda | C. Soria | R.M. Pérez-Jiménez | T. Zea | M. Talavera | F. Romero | B. De Los Santos | J.M. Vega | J.I. Páez | J. Bascón | F.J. Domínguez | P. Palencia | J.J. Medina
E. García-Méndez | D. García-Sinovas | M.A. Andrade | A. De Cal | P. Melgarejo | T. Salto | M.L. Martínez-Beringola | C. Redondo | A. Martínez-Treceño | M. Becerril | J.J. Medina | C. Soria | J.M. López-Aranda
M.G. Palha | J.L. Campo | T. Curado | M.B. Sousa | P.B. Oliveira
H.S. Yoon | Y.H. Hwang | C.G. An | J.S. Shim | H.J. Hwang | H.Y. Shin
S. López-Galarza | A. San Bautista | A. Martínez | B. Pascual | J.V. Maroto