Articles
ESTABLISHING A PPV ISOLATE COLLECTION AS A PREREQUISITE FOR AN EFFECTIVE SELECTION PROCESS IN BREEDING PLUMS FOR DURABLE RESISTANCE AGAINST SHARKA
Article number
874_6
Pages
59 – 62
Language
English
Abstract
To aid the plum breeding program for Plum pox virus (PPV) resistance at Weihenstephan a PPV isolate collection was built up.
The collection is a prerequisite for establishing a selection procedure for cultivars which are completely resistant against the Sharka disease caused by PPV. Grafted plants of the most promising seedlings of the breeding program are infected with all the isolates of the collection to test their resistance against all PPV isolates.
Up until now, 48 isolates have been collected originating from Europe, North America and Africa.
They have been classified with one of the known PPV strains (PPV-D, PPV-M, PPV-Rec, PPV-C, PPV-EA and PPV-W) using two complementary PCR protocols.
The collection is a prerequisite for establishing a selection procedure for cultivars which are completely resistant against the Sharka disease caused by PPV. Grafted plants of the most promising seedlings of the breeding program are infected with all the isolates of the collection to test their resistance against all PPV isolates.
Up until now, 48 isolates have been collected originating from Europe, North America and Africa.
They have been classified with one of the known PPV strains (PPV-D, PPV-M, PPV-Rec, PPV-C, PPV-EA and PPV-W) using two complementary PCR protocols.
Authors
J. Hadersdorfer, M. Neumüller, T. Fischer, D. Treutter
Keywords
Prunus domestica, Plum pox virus, strain specific primers, PCR
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