Articles
BIOCHEMICAL MARKERS: A USEFUL TOOL FOR ASSESSING GENETIC DIVERSITY IN JACKFRUIT (ARTOCARPUS HETEROPHYLLUS LAM.)
Article number
890_10
Pages
91 – 101
Language
English
Abstract
Using polyacrylamide gel electrophoresis, peroxidase and esterase isozymes were investigated from leaf tissue samples of 44 selected (superior) jackfruit genotypes.
These selected genotypes were collected following jackfruit descriptor 2000, after surveying 1500 trees in the districts of Nadia, 24 Parganas (N) and Coochbehar in West Bengal.
The selected genotypes were analyzed for isozyme variation for peroxidase and esterase.
Polymorphism was observed in both the enzyme systems studied, where several bands were found showing similarities and dissimilarities among the selected types.
In the peroxidase assay, 7 loci were identified among the 44 genotypes.
The relative mobility (Rm) values of the loci ranged between 0.29 and 0.50. Whereas in the esterase assay, 6 loci were identified and the relative mobility (Rm) values of the loci ranged between 0.22 and 0.65. Moreover, it was observed that genotypes collected from nearby locations did not show similar banding patterns, indicating that forces other than geographical origin, such as exchange of genetic stocks, genetic drift, spontaneous variation, natural and artificial selection, cross pollination and seedling origin of the plant are responsible for the genetic diversity.
These selected genotypes were collected following jackfruit descriptor 2000, after surveying 1500 trees in the districts of Nadia, 24 Parganas (N) and Coochbehar in West Bengal.
The selected genotypes were analyzed for isozyme variation for peroxidase and esterase.
Polymorphism was observed in both the enzyme systems studied, where several bands were found showing similarities and dissimilarities among the selected types.
In the peroxidase assay, 7 loci were identified among the 44 genotypes.
The relative mobility (Rm) values of the loci ranged between 0.29 and 0.50. Whereas in the esterase assay, 6 loci were identified and the relative mobility (Rm) values of the loci ranged between 0.22 and 0.65. Moreover, it was observed that genotypes collected from nearby locations did not show similar banding patterns, indicating that forces other than geographical origin, such as exchange of genetic stocks, genetic drift, spontaneous variation, natural and artificial selection, cross pollination and seedling origin of the plant are responsible for the genetic diversity.
Publication
Authors
L. Wangchu, D. Singh, S.K. Mitra
Keywords
genetic diversity, isozyme, jackfruit
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