Articles
CLONING AND CHARACTERIZATION OF NINE NEW S-RNASES FROM IRANIAN ALMOND CULTIVARS
Article number
912_89
Pages
593 – 599
Language
English
Abstract
The cultivated almond is a self-incompatible species which, according to several reports, originated in Central and Southwest Asia from hybridization between wild Prunus species.
Self-incompatibility is of the gametophytic type and controlled by a multigenic complex named the S-Locus, with at least two genes expressed in the pollen and in the pistil.
The S gene of the pistil is expressed mainly in the style as ribonucleases (S-RNases), which activity causes rejection of incompatible pollen.
As other species from the Rosaceae family, almond S-RNases have five conserved regions (C1-C5) and a hypervariable region.
Within the conserved region C2, the DNA coding for almond S-RNases has a highly polymorphic intron, what has allowed the identification of the S-genotype by PCR using consensus primers.
To date 32 different S-RNase alleles, most of them conferring self-incompatibility, have been identified and characterised in almond cultivars from diverse origin.
In this study, new S-RNase alleles (named S36 to S43) have been cloned and sequenced from seven Iranian almond cultivars.
Seven of them have not previously been reported in Prunus species, while the other has already been identified in an accession of the wild species Prunus webbii. Although further work regarding self-pollination tests to verify their identity as self-incompatibility alleles is needed, the information provided here is useful for breeding programmes aiming to use this material as progenitors.
Self-incompatibility is of the gametophytic type and controlled by a multigenic complex named the S-Locus, with at least two genes expressed in the pollen and in the pistil.
The S gene of the pistil is expressed mainly in the style as ribonucleases (S-RNases), which activity causes rejection of incompatible pollen.
As other species from the Rosaceae family, almond S-RNases have five conserved regions (C1-C5) and a hypervariable region.
Within the conserved region C2, the DNA coding for almond S-RNases has a highly polymorphic intron, what has allowed the identification of the S-genotype by PCR using consensus primers.
To date 32 different S-RNase alleles, most of them conferring self-incompatibility, have been identified and characterised in almond cultivars from diverse origin.
In this study, new S-RNase alleles (named S36 to S43) have been cloned and sequenced from seven Iranian almond cultivars.
Seven of them have not previously been reported in Prunus species, while the other has already been identified in an accession of the wild species Prunus webbii. Although further work regarding self-pollination tests to verify their identity as self-incompatibility alleles is needed, the information provided here is useful for breeding programmes aiming to use this material as progenitors.
Authors
E. Ortega, F. Dicenta, A. Mousavi
Keywords
Prunus dulcis, self-incompatibility, stylar ribonucleases, sequencing
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