STUDY OF POLLINATION AND SELF-INCOMPATIBILITY IN THE IRANIAN ALMOND CULTIVAR ‘MAMAEI’

‘Mamaei’ is an important commercial almond in Iran, in which self-incompatibility has been studied by classical and molecular methods.
For the classical method, the experiment was carried out by a completely randomized blocks design (CRBD) with three replications.
For each sample, fruit set following self and cross-pollination treatments was evaluated.
The percentage of final fruit set was 0% after self-pollination, while values of 16%, 17%, 19%, and 21% were determined after cross-pollination with ‘Azar’, ‘Rabie’, ‘Shahrood-21’, and ‘Sefied’ almonds, respectively.
PCR of genomic DNA from ‘Mamaei’ with the consensus primers for the second intron of S-RNases EM-PC2consFD and EM-PC3consRD, revealed the presence of two DNA fragments of sizes around 850 bp and 1250 bp on agarose gels.
The size of the smaller fragment is similar to that of S₂₅ almond RNase, while the size of the other fragment is different from all S₁-S₃₀ RNase alleles previously characterized with these primers.

V International Symposium on Pistachios and Almonds

A. Mousavi , R. Fatahi , Z. Zamani, R. Babadaei, M. Mostafavi, F. Dicenta , E. Ortega

Prunus dulcis, pollen tube growth, fruit set, PCR, S-RNase alleles

https://doi.org/10.17660/ActaHortic.2011.912.92