Articles
MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF IRANIAN ALMOND CULTIVARS AND THEIR IMPLICATIONS FOR BREEDING
Article number
912_90
Pages
601 – 606
Language
English
Abstract
In this study, genetic diversity of 41 Iranian cultivars and new releases have been studied together with 12 reference almond cultivars from different countries using morphological and molecular characterization.
To study morphological diversity, thirty-three quantitative and qualitative characteristics of nuts and kernels and almond trees were evaluated.
Cluster analysis showed that nut length, nut shape, nut weight, shell thickness and hardness, kernel length, kernel shape, kernel weight, kernel percentage, flowering time, and maturity time were the main characteristics that separated the cultivars.
Regarding the molecular characterization of the almond germplasm studied, fifteen peach simple sequence repeat (SSR) markers have been used in the molecular characterization.
DNA fingerprints have been developed establishing the genetic relatedness among cultivars and new releases.
Amplification of SSR loci was obtained for all 15 primer pairs producing polymorphic amplification.
The number of presumed alleles revealed by the SSR analysis ranged from 4 to 13 with size ranking between 75 to 205 bp.
The mean number of alleles per locus was 8.2. There were high variability and different similarity from low (0.042) to high (0.96) among the almond cultivars assayed.
Results establish the value of SSR markers for distinguishing different genetic linkages and characterize an extensive and largely underexploited Iranian germplasm available to almond breeding programs.
To study morphological diversity, thirty-three quantitative and qualitative characteristics of nuts and kernels and almond trees were evaluated.
Cluster analysis showed that nut length, nut shape, nut weight, shell thickness and hardness, kernel length, kernel shape, kernel weight, kernel percentage, flowering time, and maturity time were the main characteristics that separated the cultivars.
Regarding the molecular characterization of the almond germplasm studied, fifteen peach simple sequence repeat (SSR) markers have been used in the molecular characterization.
DNA fingerprints have been developed establishing the genetic relatedness among cultivars and new releases.
Amplification of SSR loci was obtained for all 15 primer pairs producing polymorphic amplification.
The number of presumed alleles revealed by the SSR analysis ranged from 4 to 13 with size ranking between 75 to 205 bp.
The mean number of alleles per locus was 8.2. There were high variability and different similarity from low (0.042) to high (0.96) among the almond cultivars assayed.
Results establish the value of SSR markers for distinguishing different genetic linkages and characterize an extensive and largely underexploited Iranian germplasm available to almond breeding programs.
Authors
A. Mousavi, R. Fatahi , Z. Zamani, A. Imani, F. Dicenta , P. Martínez-Gómez
Keywords
Prunus dulcis, breeding, cultivar, morphological traits, molecular markers
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