Articles
ANALYSIS OF THE VARIATION OF 18S-5.8S-28S RDNA LOCI IN ASIATIC, ORIENTAL AND LONGIFLORUM LILIES (LILIUM) USING FLUORESCENCE IN SITU HYBRIDIZATION
Article number
953_19
Pages
139 – 147
Language
English
Abstract
Diploid Lilium species and cultivars have very similar conventional karyotypes.
To be able to discriminate between them, rDNA was used as probe to analyze 15 lily cultivars using in situ hybridization.
The results indicate that there is a high degree of variation of rDNA loci among different cultivars and each lily group has its own FISH karyotype characteristics.
In Longiflorum, no 45S rDNA loci are on chromosome No. 1 and No. 2; in Oriental, there are one or two loci on No. 1 while one or two of No. 2 had no rDNA loci; in Asiatic, No. 1 has a pericentric rDNA loci and No. 2 has an interstitial loci on the short arm.
These different characteristics are a good option for distinguishing between the three lily groups.
To be able to discriminate between them, rDNA was used as probe to analyze 15 lily cultivars using in situ hybridization.
The results indicate that there is a high degree of variation of rDNA loci among different cultivars and each lily group has its own FISH karyotype characteristics.
In Longiflorum, no 45S rDNA loci are on chromosome No. 1 and No. 2; in Oriental, there are one or two loci on No. 1 while one or two of No. 2 had no rDNA loci; in Asiatic, No. 1 has a pericentric rDNA loci and No. 2 has an interstitial loci on the short arm.
These different characteristics are a good option for distinguishing between the three lily groups.
Publication
Authors
R. Guiling, K. Li, G. Zhou, Z. Xianxian, X. Tan, L. Fang, J. Jian, M. Zhu, S. Zhou
Keywords
Lilium, karyotype, FISH, rDNA
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