Articles
POLYPLOIDY INDUCTION OF THE DENDROBIUM HYBRID ‘MISS SINGAPORE’ THROUGH TISSUE CULTURE
Article number
953_25
Pages
181 – 185
Language
English
Abstract
This study was carried out to determine the optimal concentrations of colchicine and incubation time of polyploidy induction in the Dendrobium hybrid Miss Singapore through the tissue culture. Dendrobium Miss Singapore protocorms were treated with colchicine at the concentration of 0, 0.01, 0.05 and 0.1% amended in the MS medium for 1, 2, 3 and 4 days at 25±2°C under dark condition.
The results showed that the percentage of surviving plantlets decreased when the colchicine concentration and incubation time increased.
The optimal treatment was a colchicine concentration of 0.01% and incubation time of 2 days.
Only tetraploids were obtained under such condition.
The chromosome number was 2n=4x=76 as compared to the diploid control plantlets 2n=2x=38.
The results showed that the percentage of surviving plantlets decreased when the colchicine concentration and incubation time increased.
The optimal treatment was a colchicine concentration of 0.01% and incubation time of 2 days.
Only tetraploids were obtained under such condition.
The chromosome number was 2n=4x=76 as compared to the diploid control plantlets 2n=2x=38.
Publication
Authors
S. Bunnag , J. Hongthongkham
Keywords
flow cytometry, polyploidy, Dendrobium hybrid ‘Miss Singapore’, tissue culture
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