Articles
EVALUATION OF PROPAGATION METHODS BY SEEDS AND CUTTINGS IN PIPER ADUNCUM (PIPERACEAE)
Article number
964_16
Pages
129 – 134
Language
English
Abstract
Piper aduncum is a wild specie widely distributed in Sumapaz region (Cundinamarca, Colombia), which is interesting for presence of secondary metabolites. P. aduncum could be used to reduce the use of synthetic chemical products to control weeds, pests and diseases.
Its widespread use is necessary to have plant material in large quantities in order to get enough plant extract.
However, information about its propagation method is limited.
This study evaluated two methods of propagation (seeds and cuttings). In propagation by seeds viability was above 80%, germination occurred 50 days after imbibition, but seedlings had developed a stage of slow growth.
Subsequently, to induce activation of rapid growth of seedlings there were three applications of putrescine (500, 1000, 1500 µM) at intervals of a month.
After five months application of 1500 µM, resulted in greater seedling growth, which had five pairs of leaves in contrast with control and other two doses, which only formed three pairs of leaves.
Alternatively asexual propagation was assessed by cuttings in three substrates (river sand, soil, burnt rice husk) and three growth-stimulating with four different doses each (indole butyric acid (IBA), naphthalene acetic acid (NAA) and botanical extracts of aloe). Two months after preparation of cutting, the highest rooting (20 roots average) and shoot development (5 pairs of leaves) were obtained with NAA 300 and 600 mg L-1 application, with sand substrate.
The massive propagation is more efficient by cuttings, due to the slow growth stage in seedlings.
Propagation by cuttings was obtained in less time for good root development through the application of NAA, with sand substrate because this allows good drainage and maintaining a relative humidity above 80%, conditions that P. aduncum, remains in its natural growth medium.
Propagation is suggested by cuttings to get the amount of material required.
Its widespread use is necessary to have plant material in large quantities in order to get enough plant extract.
However, information about its propagation method is limited.
This study evaluated two methods of propagation (seeds and cuttings). In propagation by seeds viability was above 80%, germination occurred 50 days after imbibition, but seedlings had developed a stage of slow growth.
Subsequently, to induce activation of rapid growth of seedlings there were three applications of putrescine (500, 1000, 1500 µM) at intervals of a month.
After five months application of 1500 µM, resulted in greater seedling growth, which had five pairs of leaves in contrast with control and other two doses, which only formed three pairs of leaves.
Alternatively asexual propagation was assessed by cuttings in three substrates (river sand, soil, burnt rice husk) and three growth-stimulating with four different doses each (indole butyric acid (IBA), naphthalene acetic acid (NAA) and botanical extracts of aloe). Two months after preparation of cutting, the highest rooting (20 roots average) and shoot development (5 pairs of leaves) were obtained with NAA 300 and 600 mg L-1 application, with sand substrate.
The massive propagation is more efficient by cuttings, due to the slow growth stage in seedlings.
Propagation by cuttings was obtained in less time for good root development through the application of NAA, with sand substrate because this allows good drainage and maintaining a relative humidity above 80%, conditions that P. aduncum, remains in its natural growth medium.
Propagation is suggested by cuttings to get the amount of material required.
Authors
C. Mendoza-F., A. Celis-F., M.E. Pachón-S.
Keywords
sexual propagation, asexual propagation, polyamines, auxins, substrates, promising species
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