Articles
TOPICAL ANTI-NOCICEPTIVE EFFECT OF ARTEMISIA ABSINTHIUM EXTRACT IN MALE MICE
Article number
964_31
Pages
259 – 264
Language
English
Abstract
Traditional medicine has used medical plants to relief pain without any noticeable side effects.
It, therefore, can introduce novel drugs with less complications and costs.
In this research we evaluated the topical anti-nociceptive effect of Artemisia absinthium. This study was conducted on male mice in three groups receiving three different doses of Artemisia absinthium acidic extract, a positive control group (morphine 10 mg/ml) and a control group (acetic acid) by tail-flick method.
According to our pilot experiment three doses of Artemisia absinthium extract were chosen as 1, 2.5, 4%. The latencies of the tail-flick responses were converted to the percentage of Maximum Possible Effect (MPE %) for each time and dose according to this formula: [(test latency-control latency)/(10-control latency)]*100. According to our findings there are significant differences between control and study groups in approximately most of the different time points which means wormwood extract induced a topical anti-nociception.
The doses of 1, 2.5 and 4% in comparison with morphine (10 mg/dl) in 0, 10, 20, 30, 60 and 120 min do not show significant effect differences but had more MPE %. These results show relative equal analgesic effects among them.
In conclusion topical analgesic effect of Artemisia absinthium extract in doses of 1, 2.5 and 4% is relatively equal to morphine.
We recommend that standard human researches be done regarding wormwood recent application in traditional medicine.
It, therefore, can introduce novel drugs with less complications and costs.
In this research we evaluated the topical anti-nociceptive effect of Artemisia absinthium. This study was conducted on male mice in three groups receiving three different doses of Artemisia absinthium acidic extract, a positive control group (morphine 10 mg/ml) and a control group (acetic acid) by tail-flick method.
According to our pilot experiment three doses of Artemisia absinthium extract were chosen as 1, 2.5, 4%. The latencies of the tail-flick responses were converted to the percentage of Maximum Possible Effect (MPE %) for each time and dose according to this formula: [(test latency-control latency)/(10-control latency)]*100. According to our findings there are significant differences between control and study groups in approximately most of the different time points which means wormwood extract induced a topical anti-nociception.
The doses of 1, 2.5 and 4% in comparison with morphine (10 mg/dl) in 0, 10, 20, 30, 60 and 120 min do not show significant effect differences but had more MPE %. These results show relative equal analgesic effects among them.
In conclusion topical analgesic effect of Artemisia absinthium extract in doses of 1, 2.5 and 4% is relatively equal to morphine.
We recommend that standard human researches be done regarding wormwood recent application in traditional medicine.
Authors
M. Shams, F. Zeraati, M. Araghchian, S. Sadeghzadeh, S. Torabian, K. Razzaghi
Keywords
Artemisia absinthium, morphine, wormwood, tail flick
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