Articles
ANTIOXIDANT ACTIVITY AND QUANTITATIVE COMPOSITION OF EXTRACTS OF PIPER SPECIES FROM GUATEMALA WITH POTENTIAL USE IN NATURAL PRODUCT INDUSTRY
Article number
964_9
Pages
77 – 84
Language
English
Abstract
There is growing evidence that many diseases are caused by oxidative processes, contributing to the etiology of chronic and degenerative diseases.
Antioxidant activity is also interesting in industry for food conservation and cosmetics. Piper is an important tropical genus with high chemical and biological diversity; one of the important molecules is piperine, isolated from P. nigrum. Leaves and roots of 11 native species (P. amalago, P. hispidum, P. jacquemontianum, P. oradendron, P. patulum, P. psilorhachis, P. retalhuleuense, P. schippianum, P. sempervirens, P. umbellatum and P. variabile) were collected.
Dry extracts with dichloromethane and methanol were prepared by percolation.
Antioxidant activity was evaluated by total phenolics, DPPH, and ABTS. Phytochemical screening was performed, and by spectrophotometry UV/Vis, piperine, and flavonoids were quantified.
Important antioxidant activity was found in the methanol extract of leaves of P. psilorhachis, P. schippianum and P. variabile, which were positive by the three methods.
The methanol extracts of P. oradendron showed activity only by ABTS, but both leaves and roots.
TLC and UV/Vis procedures were established to assess the presence and amount of piperine.
The 17 extracts showing similar bands to P. nigrum by TLC were analyzed.
The highest concentrations were in P. jacquemontianum, P. retalhuleuense and P. amalago, with contents higher than P. nigrum standard.
By TLC all extracts contained flavonoids, the methanol extract of P. variabile had the highest number of bands, but quantification gave only 0.38%; the greatest amount was demonstrated in the leaves of P. oradendron (1.57%) and P. hispidum (0.87%). It is concluded that at least three of the extract have an important antioxidant activity that could be applied by the natural product industry.
Antioxidant activity is also interesting in industry for food conservation and cosmetics. Piper is an important tropical genus with high chemical and biological diversity; one of the important molecules is piperine, isolated from P. nigrum. Leaves and roots of 11 native species (P. amalago, P. hispidum, P. jacquemontianum, P. oradendron, P. patulum, P. psilorhachis, P. retalhuleuense, P. schippianum, P. sempervirens, P. umbellatum and P. variabile) were collected.
Dry extracts with dichloromethane and methanol were prepared by percolation.
Antioxidant activity was evaluated by total phenolics, DPPH, and ABTS. Phytochemical screening was performed, and by spectrophotometry UV/Vis, piperine, and flavonoids were quantified.
Important antioxidant activity was found in the methanol extract of leaves of P. psilorhachis, P. schippianum and P. variabile, which were positive by the three methods.
The methanol extracts of P. oradendron showed activity only by ABTS, but both leaves and roots.
TLC and UV/Vis procedures were established to assess the presence and amount of piperine.
The 17 extracts showing similar bands to P. nigrum by TLC were analyzed.
The highest concentrations were in P. jacquemontianum, P. retalhuleuense and P. amalago, with contents higher than P. nigrum standard.
By TLC all extracts contained flavonoids, the methanol extract of P. variabile had the highest number of bands, but quantification gave only 0.38%; the greatest amount was demonstrated in the leaves of P. oradendron (1.57%) and P. hispidum (0.87%). It is concluded that at least three of the extract have an important antioxidant activity that could be applied by the natural product industry.
Authors
A. Cáceres, S.M. Cruz, I. Gaitán, K. Guerrero, L.E. Álvarez, M.N. Marroquín
Keywords
Piper variabile, Piper psilorhachis, Piper retalhuleuense, Piper jacquemontianum, Piper oradendron, Piper schippianum
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