Articles
ANTINOCICEPTIVE ACTIVITIES OF HYPTIS CRENATA POHL
Article number
972_13
Pages
105 – 110
Language
English
Abstract
The chemical profile and medicinal effects of H. crenata on two mouse models were evaluated in order to quantify pharmacological effects caused by bioactive compounds.
A survey was conducted regarding traditional preparation and use of H. crenata in Brazil (20 regular users). The extraction methods reported were: (i) decoction (boiling in water); (ii) infusion (tea); (iii) cold extraction (water); (iv) cold extraction (15% alcohol); and (v) cold extraction (40% alcohol). The decoction extract (i) showed the highest concentration of dry matter (4.55 g/L). The traditional uses most frequently reported were for forms of mild pain (11/20 – headache, stomach discomfort, menstrual pain) or treatment of flu/fever (6/20). Based on this traditional use, a study was conducted on the antinociceptive effects of the decoction extract (i) in 8 C57/BL6 mice per treatment.
Orally administered doses of 15 and 150 mg/kg b.w. were compared with water and Indomethacin 10 mg/kg b.w in models of phasic pain (Hargreaves test) and tonic pain (acetic acid-induced writhing). In both models the treatment effects were significantly different from the water control treatment, but not from the positive control Indomethacin.
HPLC analysis of the decoction extract compared with an authentic standard of salicylic acid showed that salicylic acid was not detectable in the extract.
A survey was conducted regarding traditional preparation and use of H. crenata in Brazil (20 regular users). The extraction methods reported were: (i) decoction (boiling in water); (ii) infusion (tea); (iii) cold extraction (water); (iv) cold extraction (15% alcohol); and (v) cold extraction (40% alcohol). The decoction extract (i) showed the highest concentration of dry matter (4.55 g/L). The traditional uses most frequently reported were for forms of mild pain (11/20 – headache, stomach discomfort, menstrual pain) or treatment of flu/fever (6/20). Based on this traditional use, a study was conducted on the antinociceptive effects of the decoction extract (i) in 8 C57/BL6 mice per treatment.
Orally administered doses of 15 and 150 mg/kg b.w. were compared with water and Indomethacin 10 mg/kg b.w in models of phasic pain (Hargreaves test) and tonic pain (acetic acid-induced writhing). In both models the treatment effects were significantly different from the water control treatment, but not from the positive control Indomethacin.
HPLC analysis of the decoction extract compared with an authentic standard of salicylic acid showed that salicylic acid was not detectable in the extract.
Authors
G.S. Rocha, J. Roughan, M. Leach, P. Flecknell, C.D. Ingram, K. Brandt
Keywords
pain relief, Lamiaceae, aqueous extract
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