Articles
GENETIC TRANSFORMATION OF JAPANESE PERSIMMON WITH FLOWERING LOCUS T (FT) GENE AND TERMINAL FLOWER 1 (TFL1) HOMOLOGUES GENE
Article number
996_20
Pages
159 – 164
Language
English
Abstract
In this study, we transformed the Japanese persimmon (Diospyros kaki, Thunb. Jiro) with Arabidopsis FT gene (AtFT) and PmTFL1 gene, a Prunus mume ortholog of Arabidopsis TFL1 gene. 10 lines of transgenic PmTFL1 shoot and 2 lines of transgenic AtFT shoot were obtained.
T-DNA integrations into the Japanese persimmon genome were confirmed by PCR and DNA blot analysis.
DNA blot analysis of the PmTFL1 transgenic shoots indicated that the transformed plants carried 1-2 copies of foreign target gene.
The expression of AtFT and PmTFL1 gene in the transgenic Jiro shoots were detected by RT-PCR. The PmTFL1 transgenic in vitro shoots did not show a different appearance compared with non transformed Jiro shoots, however, the AtFT transgenic shoots showed a bushy phenotype with the short internodes.
Although the AtFT transgenic shoots were sub-cultured in vitro over 2 years, early flowering was not observed.
T-DNA integrations into the Japanese persimmon genome were confirmed by PCR and DNA blot analysis.
DNA blot analysis of the PmTFL1 transgenic shoots indicated that the transformed plants carried 1-2 copies of foreign target gene.
The expression of AtFT and PmTFL1 gene in the transgenic Jiro shoots were detected by RT-PCR. The PmTFL1 transgenic in vitro shoots did not show a different appearance compared with non transformed Jiro shoots, however, the AtFT transgenic shoots showed a bushy phenotype with the short internodes.
Although the AtFT transgenic shoots were sub-cultured in vitro over 2 years, early flowering was not observed.
Publication
Authors
M. Gao (Takai), H. Takeishi, A. Katayama, R. Tao
Keywords
Agrobacterium-mediated transformation, FT gene, Diosypyros kaki, early flowering, TFL1 gene
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