Articles
Bacillus subtilis and Trichoderma harzianum control postharvest pathogens of strawberry fruits in vitro
Article number
1117_29
Pages
181 – 184
Language
English
Abstract
This research assessed the efficacy of Bacillus subtilis (Bs) and Trichoderma harzianum (Th) (antibiosis, parasitism and volatile compounds production) for the control of important postharvest strawberry (Fragaria × ananassa) pathogens, including Rhizopus stolonifer (Rs) (soft rot), Botrytis cinerea (Bc) (gray mold), and Colletotrichum spp. (Col) (anthracnose), in paired cultures.
Those are etiologic agents of major strawberry (Fragaria × ananassa) postharvest diseases.
Commercial available antagonists, and pathogens isolated from infected strawberry fruit were used.
Pathogens were paired in petri dishes containing potato dextrose agar (PDA) with Bs aliquots of 80, 60, 40 and 20 µL plate-1 or Th aliquots of 62.50, 46.88, 31.25 and 16.60 µL plate-1. Plates were incubated at 25±1°C, photoperiod of 12 h.
The Bs 80 µL aliquot reduced mycelial growth of Bc and Col at 90 and 98%, respectively. Th did not inhibit growth of Rs. Bs and Th produced no volatile compounds in petri dishes able to inhibit the pathogens.
The inhibition zones and the reduced mycelial growth of pathogens showed that Bs and Th acted by antibiosis.
These results indicate the potential of Bs and Th for postharvest strawberry diseases control.
Those are etiologic agents of major strawberry (Fragaria × ananassa) postharvest diseases.
Commercial available antagonists, and pathogens isolated from infected strawberry fruit were used.
Pathogens were paired in petri dishes containing potato dextrose agar (PDA) with Bs aliquots of 80, 60, 40 and 20 µL plate-1 or Th aliquots of 62.50, 46.88, 31.25 and 16.60 µL plate-1. Plates were incubated at 25±1°C, photoperiod of 12 h.
The Bs 80 µL aliquot reduced mycelial growth of Bc and Col at 90 and 98%, respectively. Th did not inhibit growth of Rs. Bs and Th produced no volatile compounds in petri dishes able to inhibit the pathogens.
The inhibition zones and the reduced mycelial growth of pathogens showed that Bs and Th acted by antibiosis.
These results indicate the potential of Bs and Th for postharvest strawberry diseases control.
Authors
F.C. Ayduki, L.C. Rozwalka, M.A.C. Zawadneak, F.L. Cuquel
Keywords
biological control, antagonistic microorganisms, Rhizopus stolonifer, Botrytis cinerea, Colletotrichum spp
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