UPTAKE AND TRANSLOCATION OF ¹⁴C-CHLOROETHYL LABELED (2-CHLOROETHYL) METHYLBIS(PHENYLMETHOXY) SILANE, (CGA-15281), IN THE PEACH

(2-chloroethyl)methylbis(phenylmethoxy)silane (Fig. 1) has shown exceptional promise as an ethylene releasing chemical for peach thinning due to the temperature stability of its ethylene release kinetics (apparent energy of activation = 10 Kcal mole^-1). Fruits and shoots of ‘Bicentennial’ peaches were individually treated with 50 μl of a 600 ppm solution of parent compound labeled in the chloroethyl position (Sp.
Act. 15 m Ci/mM; 665,000 CPM/ application) with appropriate surfactants.
Treated fruit, leaves, and shoots were sampled 0, 4, 8, 48 and 96 hrs following application.
Fruit sampling consisted of the removal of a 9 mm core of mesocarp tissue which was cut into 1 mm sections beginning from the inner to the outer fruit surface.
The shoot was cut at the treated mid-leaf and divided into acropetal and basipetal sections.
Each shoot portion was divided into 1 mm sections which were retained in the sequence of removal from the shoot.
The treated mid-leaf was collected and retained for analysis.

Figure 1 (2-chloroethyl)methylbis (phenylmethoxy) silane
Formula available in full text only

Since the chloroethyl bond is the most labile, the presence of ¹⁴C-ethylene formed upon hydrolysis by 6 ml of 1 N NaOH was used as a measure of the amount of intact parent molecule.
Samples were stored at -10°C until measured allowing the dissipation of gaseous ¹⁴C-ethylene from within the tissue before assaying.
Samples were sliced in small pieces with a razor and placed in sealed 125 ml Erlenmyer flasks containing 2 ml of mecuric acetate in a small test tube.
NaOH was introduced to the tissue through the serum vile stopper sealing the flask.
After 24 hr the mecuric acetate was transferred to scintillation viles containing 20 ml of dioxane and counted.

Symposium on Growth Regulators in Fruit Production

G.A. Couvillon, S.J. Kays, S.D. Seeley

https://doi.org/10.17660/ActaHortic.1981.120.34