Articles
Molecular characterization of ten Heliconia (Heliconiaceae) genotypes by means of RAPD markers
Article number
1288_14
Pages
95 – 102
Language
English
Abstract
Heliconia a trendiest specialty cut flower of tropics is gaining popularity for its inflorescences charismatic form and alluringly blended hues in the world and in Indian market too.
It is a monophyletic genus with roughly 200 to 250 species of neotropical origin.
It resides in the Heliconiaceae family of Zingiberales order former incorporated in Musaceae for fusion of inverted flowers, single staminode and drupe type fruits elite for it.
Exploitation of molecular markers may aid in perceptive recognition of several species and cultivars, since taxonomic confusions and uncertainties subsist among them.
In this context, ten species and cultivars were analyzed using RAPD markers.
Thirty primers among 70 were chossen, which amplified 1281 polymorphic DNA fragments with each primer giving a mean of 42.7 polymorphic bands.
The genetic similarity matrix constructed with Jaccards coefficient using RAPD marker scores showed that the highest value was between H. psittacorum Choconiana and H. psittacorum Lady Di (0.384), while the lowest was between H. psittacorum Choconiana and H. stricta Dwarf Jamaican Red (0.244). Ten species and cultivars of Heliconia formed three distinct clusters at similarity coefficient value of 0.33, implying a parallelism between genetic and morphologic or taxonomic variability of Heliconia genotypes. Golden Torch, Choconiana, Lady Di and H. wagneriana included in cluster I while Choconiana, Lady Di formed a more cohesive entity.
On the contrary, H. stricta var. Dwarf Jamaican Red produced a separate cluster validating that taxonomically related entries clustered together and distant ones segregated.
So, the current sequels demonstrate that RAPD is a rapid, relatively economical and functional for the characterization of genetic divergence between different Heliconia genotypes.
It is a monophyletic genus with roughly 200 to 250 species of neotropical origin.
It resides in the Heliconiaceae family of Zingiberales order former incorporated in Musaceae for fusion of inverted flowers, single staminode and drupe type fruits elite for it.
Exploitation of molecular markers may aid in perceptive recognition of several species and cultivars, since taxonomic confusions and uncertainties subsist among them.
In this context, ten species and cultivars were analyzed using RAPD markers.
Thirty primers among 70 were chossen, which amplified 1281 polymorphic DNA fragments with each primer giving a mean of 42.7 polymorphic bands.
The genetic similarity matrix constructed with Jaccards coefficient using RAPD marker scores showed that the highest value was between H. psittacorum Choconiana and H. psittacorum Lady Di (0.384), while the lowest was between H. psittacorum Choconiana and H. stricta Dwarf Jamaican Red (0.244). Ten species and cultivars of Heliconia formed three distinct clusters at similarity coefficient value of 0.33, implying a parallelism between genetic and morphologic or taxonomic variability of Heliconia genotypes. Golden Torch, Choconiana, Lady Di and H. wagneriana included in cluster I while Choconiana, Lady Di formed a more cohesive entity.
On the contrary, H. stricta var. Dwarf Jamaican Red produced a separate cluster validating that taxonomically related entries clustered together and distant ones segregated.
So, the current sequels demonstrate that RAPD is a rapid, relatively economical and functional for the characterization of genetic divergence between different Heliconia genotypes.
Authors
M. Malakar, P. Acharyya, S. Biswas
Keywords
Heliconia, DNA, molecular characterization, RAPD, cluster
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