Articles
The orthologous Flowering Locus T (FT) and LEAFY (LFY) genes in the floral transition of Polianthes tuberosa
Article number
1288_34
Pages
225 – 230
Language
English
Abstract
The tuberose (Polianthes tuberosa L.) is an endemic plant from Mexico that belongs to the family Asparagaceae, which for its beauty and fragrance is used as a cut flower or for the perfumery industry.
Although it is a species cultivated in different parts of the world, the literature regarding the forcing and the morphological and biochemical changes during the floral transition is minimal and with contradictory results; also studies in tuberose at the level of gene expression are limited.
The objective of this work was to isolate and identify the FT and LFY orthologous genes in P. tuberosa. For which we proceeded to the generation of cDNA sequences using degenerate primers with the purpose of obtaining partial sequences of the orthologs of FT and LFY (550 and 350 bp, respectively), later the complementary regions were obtained by the RACE technique.
The degree of homology with respect to previous reports with other related geophytes, as well as the potential use of these P. tuberosa orthologous in the evaluation of their expression during the floral transition will be discussed.
Although it is a species cultivated in different parts of the world, the literature regarding the forcing and the morphological and biochemical changes during the floral transition is minimal and with contradictory results; also studies in tuberose at the level of gene expression are limited.
The objective of this work was to isolate and identify the FT and LFY orthologous genes in P. tuberosa. For which we proceeded to the generation of cDNA sequences using degenerate primers with the purpose of obtaining partial sequences of the orthologs of FT and LFY (550 and 350 bp, respectively), later the complementary regions were obtained by the RACE technique.
The degree of homology with respect to previous reports with other related geophytes, as well as the potential use of these P. tuberosa orthologous in the evaluation of their expression during the floral transition will be discussed.
Authors
J.C. Fragoso-Jimenez, D.E. Navarro-Lopez, R. Barba-Gonzalez, M.C. Castañeda-Saucedo, E. Tapia-Campos
Keywords
bulbous ornamental, forcing, gene expression
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