Articles
Discernment of pollen grain fertility and stigma receptivity applying Hasty-Easy techniques of Heliconia L. species under optical microscope
Article number
1288_21
Pages
139 – 148
Language
English
Abstract
Evaluation of pollen grain fertility and stigma receptivity are an essential pre-requisite for breeding program of Heliconia sp.
Acetocarmine staining method adopted for pollen fertility and stigma receptivity by Bakers procedure, 6% hydrogen peroxide (H2O2) and Macherey-Nagel Peroxtesmo Ko peroxidase test papers on Heliconia psittacorum × spathocircinata Golden torch, Heliconia psittacorum Choconiana, Heliconia psittacorum Lady Di, Heliconia rostrata, Heliconia humilis, Heliconia humilis Dwarf, Heliconia wagneriana, Heliconia stricta and Heliconia metallica cultivated at the Agricultural Experimental Farm, Baruipur of University of Calcutta were accomplished.
Samples consisted of five mature anthers and 30 freshly cut stigmas at different stages of flower development of each species.
Utmost pollen fertility scrutinized under Magnüs MLX Plus at 10 and 40× augmentations as exhibited by Choconiana (100%), Lady Di (94%) and H. metallica (93%) and H. humilis (91.87%) along with sizeable pollens ranged from 581.11 to 392.25 µm while unstained, sterilepollens of Golden torch and H. stricta were significantly smaller sized.
Prominently stained stigma tips by Bakers and Peroxtesmo tests were perceived in all taxa.
Thus, Golden torch and H. stricta as female parents and others as pollen parents can be utilized for hybridization.
Acetocarmine staining method adopted for pollen fertility and stigma receptivity by Bakers procedure, 6% hydrogen peroxide (H2O2) and Macherey-Nagel Peroxtesmo Ko peroxidase test papers on Heliconia psittacorum × spathocircinata Golden torch, Heliconia psittacorum Choconiana, Heliconia psittacorum Lady Di, Heliconia rostrata, Heliconia humilis, Heliconia humilis Dwarf, Heliconia wagneriana, Heliconia stricta and Heliconia metallica cultivated at the Agricultural Experimental Farm, Baruipur of University of Calcutta were accomplished.
Samples consisted of five mature anthers and 30 freshly cut stigmas at different stages of flower development of each species.
Utmost pollen fertility scrutinized under Magnüs MLX Plus at 10 and 40× augmentations as exhibited by Choconiana (100%), Lady Di (94%) and H. metallica (93%) and H. humilis (91.87%) along with sizeable pollens ranged from 581.11 to 392.25 µm while unstained, sterilepollens of Golden torch and H. stricta were significantly smaller sized.
Prominently stained stigma tips by Bakers and Peroxtesmo tests were perceived in all taxa.
Thus, Golden torch and H. stricta as female parents and others as pollen parents can be utilized for hybridization.
Authors
M. Malakar, P. Acharyya, S. Biswas
Keywords
Heliconia, pollen, stigma, fertility, receptivity, microscope
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