Articles
Obtaining polyploids in wild species of the genus Polianthes
Article number
1288_9
Pages
65 – 70
Language
English
Abstract
In the present work the effect of different times and concentrations of colchicine to get polyploid plants from four wild species of the genus Polianthes (P. montana, P. pringlei, P. platyphylla and P. zapopanensis) was evaluated.
Diploid in vitro seedlings (2n=2x=60) were immersed in six colchicine concentrations (0, 0.001, 0.01, 0.05, 0.1 and 0.2%) with two exposure times (24 and 48 h). The survival of the explants, the number of buds produced and the height of shoots were evaluated.
Some seedlings showed necrosis in the concentration of 0.2%, however, 100% survival was achieved.
Both the number of shoots and shoot height showed mean values inversely proportional to the colchicine concentration.
The chromosome counts from roots revealed that 0.2% of colchicine was the most accurate concentration to obtain poliploids in Polianthes since in the four treated species at least one tetraploid was obtained (2n=4x=120) with this concentration.
In addition, in P. montana an octaploid was obtained (2n=8x=240). Regarding the submersion time, there were no differences in the effect at 24 and 48 h, except in P. platyphylla, which only obtained a tetraploid at 48 h with 0.2% colchicine.
Such results will serve as a reference to lead to more efficient protocols in obtaining polyploids which can be used in Polianthes breeding.
Diploid in vitro seedlings (2n=2x=60) were immersed in six colchicine concentrations (0, 0.001, 0.01, 0.05, 0.1 and 0.2%) with two exposure times (24 and 48 h). The survival of the explants, the number of buds produced and the height of shoots were evaluated.
Some seedlings showed necrosis in the concentration of 0.2%, however, 100% survival was achieved.
Both the number of shoots and shoot height showed mean values inversely proportional to the colchicine concentration.
The chromosome counts from roots revealed that 0.2% of colchicine was the most accurate concentration to obtain poliploids in Polianthes since in the four treated species at least one tetraploid was obtained (2n=4x=120) with this concentration.
In addition, in P. montana an octaploid was obtained (2n=8x=240). Regarding the submersion time, there were no differences in the effect at 24 and 48 h, except in P. platyphylla, which only obtained a tetraploid at 48 h with 0.2% colchicine.
Such results will serve as a reference to lead to more efficient protocols in obtaining polyploids which can be used in Polianthes breeding.
Authors
M.J. Ferrer-Cervantes, J.M. Rodríguez-Domínguez, R. Barba-Gonzalez, M.C. Castañeda-Saucedo, E. Tapia-Campos
Keywords
ornamental geophytes, plant breeding, tissue culture, colchicine
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