Articles
Effects of preharvest UV-B irradiation on anthocyanin biosynthesis in blueberry
Article number
1312_21
Pages
139 – 144
Language
English
Abstract
The effects of preharvest UV irradiation on blueberry fruit development were investigated to explore the potential use of UV irradiation in blueberry production systems.
Long-term UV-B treatments with environment-dependent dosing were applied to southern highbush blueberry (Vaccinium corymbosum interspecific hybrid), ‘O’Neal’ trees in a greenhouse, during the entire reproductive period.
The experimental treatments included UV irradiation conditions representing an average winter day (low, 0.07 W m‑2), an average summer day (medium, 0.14 W m‑2) and 30% above that of an average summer day (high, 0.19 W m‑2). Compared with the control, the total anthocyanin content increased by 167 and 148% after the application of medium- and high-dose UV, respectively.
Gene expression changes of anthocyanin biosynthesis-related structure genes, the MYB transcription factor, a UV receptor, and light-responsive genes were examined and different responses under different UV-B exposures were identified.
Simultaneous upregulation of the MYB transcription factor, VcMYBPA1, with biosynthesis-related structure genes (such as VcCHS and VcDFR) in response to low- and high-dose UV-B suggests that VcMYBPA1 may increase biosynthesis-related gene expression, thereby enhancing anthocyanin accumulation in fruits in response to UV-B. The results demonstrate the possible use of UV-B irradiation at doses of 0.14 and 0.19 W m‑2 to improve blueberry anthocyanin accumulation.
Long-term UV-B treatments with environment-dependent dosing were applied to southern highbush blueberry (Vaccinium corymbosum interspecific hybrid), ‘O’Neal’ trees in a greenhouse, during the entire reproductive period.
The experimental treatments included UV irradiation conditions representing an average winter day (low, 0.07 W m‑2), an average summer day (medium, 0.14 W m‑2) and 30% above that of an average summer day (high, 0.19 W m‑2). Compared with the control, the total anthocyanin content increased by 167 and 148% after the application of medium- and high-dose UV, respectively.
Gene expression changes of anthocyanin biosynthesis-related structure genes, the MYB transcription factor, a UV receptor, and light-responsive genes were examined and different responses under different UV-B exposures were identified.
Simultaneous upregulation of the MYB transcription factor, VcMYBPA1, with biosynthesis-related structure genes (such as VcCHS and VcDFR) in response to low- and high-dose UV-B suggests that VcMYBPA1 may increase biosynthesis-related gene expression, thereby enhancing anthocyanin accumulation in fruits in response to UV-B. The results demonstrate the possible use of UV-B irradiation at doses of 0.14 and 0.19 W m‑2 to improve blueberry anthocyanin accumulation.
Publication
Authors
L. Taishan, H. Yamane, R. Tao
Keywords
anthocyanin, blueberry, fruit maturation, MYB, UV-B
Groups involved
- Division Temperate Tree Fruits
- Division Vine and Berry Fruits
- Division Tropical and Subtropical Fruit and Nuts
- Division Ornamental Plants
- Division Vegetables, Roots and Tubers
- Division Horticulture for Human Health
- Division Temperate Tree Nuts
- Division Precision Horticulture and Engineering
- Division Plant Genetic Resources, Breeding and Biotechnology
- Division Sustaining Horticulture in a Changing World
- Division Plant-Environment Interactions in Field Systems
- Division Postharvest and Quality Assurance
- Division Greenhouse and Indoor Production Horticulture
- Division Landscape and Urban Horticulture
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