Articles
Investigating genetic control of salt stress tolerance in tomato commercial hybrid cultivars and Solanum pennellii introgression lines
Article number
1351_27
Pages
165 – 174
Language
English
Abstract
Global warming is decreasing the ratio of freshwater to saltwater.
Soil salinity is a major environmental constraint to plant growth, and it is a serious problem in agricultural systems that rely heavily on water source conditions used for irrigation (Flowers, 2004; Foolad, 2004). Plants damaged by high salinity may suffer reduced shoot and root growth, yield losses and eventual death (Xiong et al., 2002). Cultivations require cultivars with high resilience to drought and salt stresses and economically sustainable agronomical performances.
Root development will play a key role in improving adaptability of processing tomato hybrids to face extreme climatic conditions.
To highlight the differences in root development, three different concentrations of NaCl, 0, 40 and 80 mM, were tested on two commercial hybrid cultivars (Mariner F1‘ and Foster F1‘) of ISI Sementi and on UC82, one accession of S. lycopersicum. Once the effective concentrations were chosen (0 and 40 mM), they were used to test S. lycopersicum (susceptible elite parental) × S. pennellii (tolerant wild parental) introgression lines (ILs), with the purpose to identify the most tolerant accessions to salt stress.
Analysing ILs and commercial hybrid cultivars under control conditions (NaCl 0 mM) and under conditions of saline stress (NaCl 40 mM) is a viable strategy to highlight candidate QTLs and loci involved in root development in salt stress condition.
All the ILs available on TGRC were growth in tubes with MS culture media containing different concentrations (0, 40 and 80 mM) of NaCl, in a growing chamber with controlled condition to identify the concentration useful to highlight different root development of accessions (TGRC, 2020). Increasing concentrations of NaCl showed a general reduction in germination rate and root development.
The ILs population was tested with the same protocol at a concentration of 40 mM. Accession LA4068 (IL7-4-1) showed significant values of root development, suggesting an effect of chromosome 07 short-arm on salt stress tolerance.
Comparison with data obtained on accession containing introgression close or overlapped to IL7-4-1, allowed to identify the 7C locus as involved in root development in salt stress condition.
Soil salinity is a major environmental constraint to plant growth, and it is a serious problem in agricultural systems that rely heavily on water source conditions used for irrigation (Flowers, 2004; Foolad, 2004). Plants damaged by high salinity may suffer reduced shoot and root growth, yield losses and eventual death (Xiong et al., 2002). Cultivations require cultivars with high resilience to drought and salt stresses and economically sustainable agronomical performances.
Root development will play a key role in improving adaptability of processing tomato hybrids to face extreme climatic conditions.
To highlight the differences in root development, three different concentrations of NaCl, 0, 40 and 80 mM, were tested on two commercial hybrid cultivars (Mariner F1‘ and Foster F1‘) of ISI Sementi and on UC82, one accession of S. lycopersicum. Once the effective concentrations were chosen (0 and 40 mM), they were used to test S. lycopersicum (susceptible elite parental) × S. pennellii (tolerant wild parental) introgression lines (ILs), with the purpose to identify the most tolerant accessions to salt stress.
Analysing ILs and commercial hybrid cultivars under control conditions (NaCl 0 mM) and under conditions of saline stress (NaCl 40 mM) is a viable strategy to highlight candidate QTLs and loci involved in root development in salt stress condition.
All the ILs available on TGRC were growth in tubes with MS culture media containing different concentrations (0, 40 and 80 mM) of NaCl, in a growing chamber with controlled condition to identify the concentration useful to highlight different root development of accessions (TGRC, 2020). Increasing concentrations of NaCl showed a general reduction in germination rate and root development.
The ILs population was tested with the same protocol at a concentration of 40 mM. Accession LA4068 (IL7-4-1) showed significant values of root development, suggesting an effect of chromosome 07 short-arm on salt stress tolerance.
Comparison with data obtained on accession containing introgression close or overlapped to IL7-4-1, allowed to identify the 7C locus as involved in root development in salt stress condition.
Publication
Authors
G. Ferrari, S. Americo, M.L. Prazzoli, M. Beretta
Keywords
global warming, climatic change, salt stress, water stress, abiotic stress, tomato, introgression lines, roots
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