Articles
Effect of different LED wavelength absorption spectra and light intensities on in vitro shoot proliferation of GF 677, MM 106 and Gisela 6 rootstocks
Article number
1413_2
Pages
13 – 20
Language
English
Abstract
Light‐emitting diodes (LEDs) panels having emission spectra matching with the absorption spectra of plant photoreceptors may yield optimal in vitro productivity, influencing plant morphogenesis and metabolism.
In this study, three light wavelength absorption spectra including 400-700 nm (mainly blue-green, BG), 430-690 nm (mainly blue-red, BR) and 430-690 nm (blue-green-yellow-orange-red, BGYOR) in combination with three light intensities (20, 40, 120 μmol m‑2 s‑1) were used for in vitro shoot proliferation of peach GF 677, apple MM 106 and cherry Gisela 6 rootstocks.
Thus, five in total treatments: white cool fluorescent light (WFL) (BG, 40 μmol m‑2 s‑1), LED1 (BR, 40 μmol m‑2 s‑1), LED2 (BGYOR, 40 μmol m‑2 s‑1), LED3 (BR, 20 μmol m‑2 s‑1) and LED4 (BR, 120 μmol m‑2 s‑1) were tested.
The culture medium used was the MS enriched with BA (2.7, 3.1 and 4.4 μM for MM 106, GF 677 and Gisela 6), 0.05 μM NAA, 0.3 μM GA3, 20 g L‑1 sucrose (pH 5.8) and 6 g L‑1 Plant Agar (shoot nodes, 30 days, 16 h photoperiod, 22±1°C). Regardless absorption spectrum and light intensity, LED3 in MM 106 (90.48% shoot formation, 2.29 proliferation rates or shoot numbers of 1.23 cm), WFL and LED1 in Gisela 6 (100% shoot formation, 3.8-4.3 proliferation rates or shoot numbers of 0.9 cm), and WFL, LED2 and LED3 in GF 677 (90.48-96.43% shoot formation, 1.9-2.18 proliferation rates or shoot numbers of 0.44-0.52 cm) proved to be the most effective treatments, exhibiting similar results.
In all three rootstocks, LED4 negatively affected shoot proliferation raising simultaneously the percentage of explants with abiotic stress symptoms.
It is evident that the ideal light environment i.e. different wavelength absorption spectra and intensity for each rootstock is unique.
The spectral energy distribution of LEDs could satisfy the requirements of various plant tissue culture aspects including commercial micropropagation providing lower-cost, more energy- and higher-yield efficient production systems, compared to high electricity consumption/low output efficiency of the fluorescent lamps.
In this study, three light wavelength absorption spectra including 400-700 nm (mainly blue-green, BG), 430-690 nm (mainly blue-red, BR) and 430-690 nm (blue-green-yellow-orange-red, BGYOR) in combination with three light intensities (20, 40, 120 μmol m‑2 s‑1) were used for in vitro shoot proliferation of peach GF 677, apple MM 106 and cherry Gisela 6 rootstocks.
Thus, five in total treatments: white cool fluorescent light (WFL) (BG, 40 μmol m‑2 s‑1), LED1 (BR, 40 μmol m‑2 s‑1), LED2 (BGYOR, 40 μmol m‑2 s‑1), LED3 (BR, 20 μmol m‑2 s‑1) and LED4 (BR, 120 μmol m‑2 s‑1) were tested.
The culture medium used was the MS enriched with BA (2.7, 3.1 and 4.4 μM for MM 106, GF 677 and Gisela 6), 0.05 μM NAA, 0.3 μM GA3, 20 g L‑1 sucrose (pH 5.8) and 6 g L‑1 Plant Agar (shoot nodes, 30 days, 16 h photoperiod, 22±1°C). Regardless absorption spectrum and light intensity, LED3 in MM 106 (90.48% shoot formation, 2.29 proliferation rates or shoot numbers of 1.23 cm), WFL and LED1 in Gisela 6 (100% shoot formation, 3.8-4.3 proliferation rates or shoot numbers of 0.9 cm), and WFL, LED2 and LED3 in GF 677 (90.48-96.43% shoot formation, 1.9-2.18 proliferation rates or shoot numbers of 0.44-0.52 cm) proved to be the most effective treatments, exhibiting similar results.
In all three rootstocks, LED4 negatively affected shoot proliferation raising simultaneously the percentage of explants with abiotic stress symptoms.
It is evident that the ideal light environment i.e. different wavelength absorption spectra and intensity for each rootstock is unique.
The spectral energy distribution of LEDs could satisfy the requirements of various plant tissue culture aspects including commercial micropropagation providing lower-cost, more energy- and higher-yield efficient production systems, compared to high electricity consumption/low output efficiency of the fluorescent lamps.
Authors
K. Grigoriadou, V. Sarropoulou, A. Konstantinidis
Keywords
commercial micropropagation, irradiance, light‐emitting diodes, light quality, Malus domestica Borkh., photomorphogenesis, Prunus cerasus × Prunus canescens, Prunus persica × Prunus amygdalus
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