Articles
The use of the encapsulation technology as a tool of micropropagation in Sicilian hazelnut genotypes sanitized from apple mosaic virus (ApMV), for the production of certified plant material
Article number
1413_6
Pages
45 – 58
Language
English
Abstract
An efficient synthetic seed propagation protocol, based on the calcium alginate coating, was carried out for some Sicilian cultivars of hazelnut (Corylus avellana L.), previously sanitized from apple mosaic virus (ApMV), the most economically damaging Ilarvirus on a worldwide scale.
Uninodal microcuttings (3-4 mm length) were excised from in vitro sanitized proliferating shoots of three cultivars ‘Curcia’, ‘Panottara Collica’ and ‘Panottara Galati Grande’, and subjected to the encapsulation technology.
The two different basic salts in matrixes of calcium alginate Murashige and Skoog (MS) and Juglans (DKW) media, supplemented with 3 mg L‑1 6-benzylaminopurine (BAP) and 54 g L‑1 sucrose were evaluated for capsules preparation.
Rooting of encapsulated propagules was carried out on media containing MS and DKW basal salts, supplemented with 30 g L‑1 sucrose and indole-3-butyric acid (IBA) at different concentrations (0.6, 1 and 2 mg L‑1) and on media hormone-free.
Data were collected at 30- (P1) and 60- (P2) days post-sowing for all experiments, and viability, regrowth and conversion rates were registered and statistically evaluated.
The highest viability and regrowth rates were obtained after 30 days post-sowing by ‘Panottara Collica’ (88 and 43%, respectively) followed by ‘Panottara Galati Grande’ (75 and 34%, respectively) and ‘Curcia’ (47 and 23%, respectively). At P1, DKW medium, supplemented with 3 mg L‑1 of BAP (capsule) gave the highest viability (80%) and regrowth (42%) rates on the rooting media supplemented with 0.6 and 1 mg L‑1 of IBA, respectively.
Whereas, at P2, the greatest viability (66%) and regrowth (53%) rates were obtained on MS medium augmented with 2 mg L‑1 of IBA. Conversion phase was successfully achieved, exclusively on MS media containing 2 mg L 1 of IBA after 60 days post-sowing. ‘Panottara Collica’ registered the highest conversion rate (27%) followed by ‘Panottara Galati Grande’ with 20%. ‘Curcia’ didn’t succeed to develop roots in any experiment.
For the first time the conversion of the synthetic seeds for these virus-free genotypes of Sicilian hazelnuts was reported, with the success of the rooting stage in the encapsulation technology.
Uninodal microcuttings (3-4 mm length) were excised from in vitro sanitized proliferating shoots of three cultivars ‘Curcia’, ‘Panottara Collica’ and ‘Panottara Galati Grande’, and subjected to the encapsulation technology.
The two different basic salts in matrixes of calcium alginate Murashige and Skoog (MS) and Juglans (DKW) media, supplemented with 3 mg L‑1 6-benzylaminopurine (BAP) and 54 g L‑1 sucrose were evaluated for capsules preparation.
Rooting of encapsulated propagules was carried out on media containing MS and DKW basal salts, supplemented with 30 g L‑1 sucrose and indole-3-butyric acid (IBA) at different concentrations (0.6, 1 and 2 mg L‑1) and on media hormone-free.
Data were collected at 30- (P1) and 60- (P2) days post-sowing for all experiments, and viability, regrowth and conversion rates were registered and statistically evaluated.
The highest viability and regrowth rates were obtained after 30 days post-sowing by ‘Panottara Collica’ (88 and 43%, respectively) followed by ‘Panottara Galati Grande’ (75 and 34%, respectively) and ‘Curcia’ (47 and 23%, respectively). At P1, DKW medium, supplemented with 3 mg L‑1 of BAP (capsule) gave the highest viability (80%) and regrowth (42%) rates on the rooting media supplemented with 0.6 and 1 mg L‑1 of IBA, respectively.
Whereas, at P2, the greatest viability (66%) and regrowth (53%) rates were obtained on MS medium augmented with 2 mg L‑1 of IBA. Conversion phase was successfully achieved, exclusively on MS media containing 2 mg L 1 of IBA after 60 days post-sowing. ‘Panottara Collica’ registered the highest conversion rate (27%) followed by ‘Panottara Galati Grande’ with 20%. ‘Curcia’ didn’t succeed to develop roots in any experiment.
For the first time the conversion of the synthetic seeds for these virus-free genotypes of Sicilian hazelnuts was reported, with the success of the rooting stage in the encapsulation technology.
Authors
E. Yahyaoui, T. Caruso, S. Davino, M.A. Germanà
Keywords
conversion, Corylus avellana L., micropropagation, rooting, synthetic seeds, virus-free
Online Articles (28)