Articles
Micropropagation via nodal and internodal explants of Russian olive (Elaeagnus angustifolia L.) in Lebanon
Article number
1413_8
Pages
143 – 148
Language
English
Abstract
Russian olive (Elaeagnus angustifolia L.) can tolerate and survive a wide variety of environmental conditions.
In addition, it can be used in different therapeutic applications.
The propagation of this species using traditional methods is often difficult.
Therefore this study aimed to develop an efficient in vitro propagation protocol in order to overcome this difficulty.
Nodal segments and internodes sections, taken from 10-year old trees, were first sterilized before being successfully introduced in vitro for cultures establishment.
Two basic mineral compositions, Murashige and Skoog (MS) and Lloyd & McCown Woody Plant Medium (WPM), supplemented with different plant growth regulator combinations.
The medium supplemented with 1 mg L‑1 of BAP and 0.1 mg L‑1 of NAA presented the highest average number of shootlets; 1.75. Callogenesis of internodes sections was successful (83.33%) on MS medium plus 2 mg L‑1 of zeatin and 0.1 mg L‑1 of NAA. The best proliferation rate (3.81) was recorded on MS or WPM media devoid of growth regulators at the end of second subculture.
Regenerated shootlets were transferred onto 12 rooting media consisting of either full or half-strength MS or WPM macro-elements supplemented with different concentrations of IBA (0, 1, and 2 mg L‑1). Shootlet rooting was possible on full or half strength MS and WPM macro-elements without growth regulators, while the different concentrations of auxin failed to induce rooting.
Rooted shootlets were all successfully acclimatized under the greenhouse conditions.
In addition, it can be used in different therapeutic applications.
The propagation of this species using traditional methods is often difficult.
Therefore this study aimed to develop an efficient in vitro propagation protocol in order to overcome this difficulty.
Nodal segments and internodes sections, taken from 10-year old trees, were first sterilized before being successfully introduced in vitro for cultures establishment.
Two basic mineral compositions, Murashige and Skoog (MS) and Lloyd & McCown Woody Plant Medium (WPM), supplemented with different plant growth regulator combinations.
The medium supplemented with 1 mg L‑1 of BAP and 0.1 mg L‑1 of NAA presented the highest average number of shootlets; 1.75. Callogenesis of internodes sections was successful (83.33%) on MS medium plus 2 mg L‑1 of zeatin and 0.1 mg L‑1 of NAA. The best proliferation rate (3.81) was recorded on MS or WPM media devoid of growth regulators at the end of second subculture.
Regenerated shootlets were transferred onto 12 rooting media consisting of either full or half-strength MS or WPM macro-elements supplemented with different concentrations of IBA (0, 1, and 2 mg L‑1). Shootlet rooting was possible on full or half strength MS and WPM macro-elements without growth regulators, while the different concentrations of auxin failed to induce rooting.
Rooted shootlets were all successfully acclimatized under the greenhouse conditions.
Authors
A. Elbitar, Z. Chamas, A. Chehade, E. Choueiri, H. Diab, Y. Ghorra, Z. Fahs
Keywords
oleaster, in vitro culture, callogenesis, shootlets proliferation, rooting
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