Articles
Propagation of certified Fragaria ananassa in tissue culture
Article number
1413_7
Pages
59 – 66
Language
English
Abstract
The article presents the experimental data on the production of certified healthy planting material of strawberry of four cultivars: ‘San Andres’, ‘Malwina’, ‘Black Prince’ and ‘Sabrina’ in apical meristem culture.
As a result of the conducted studies, viral infection of mother plants was studied by ELISA, herbaceous indicators (for sap-transmissible viruses) and PCR. Selected strawberry cultivars were introduced into the tissue culture for improvement and propagation, with the improvement of the technological scheme of clonal micro- propagation specifically for each cultivar at the stages of explant sterilization, introduction into tissue culture, primary regeneration of apexes, proliferation and rhizogenesis with genotype preservation.
We have developed a biotechnology of in vitro micropropagation of garden strawberries and obtaining planting material free from viral and mycoplasma infections.
Explants were cultured on agarized nutrient medium at the stage of tissue culture and regeneration without growth regulators, with ATP and proline amino acid 1 mg L-1 each in the nutrient medium; at the stage of proliferation, cytokinin 6 benzylaminopurine (6BAP) was added to the nutrient medium; at the stage of rhizogenesis, kinetin and IBA (indole butyric acid) were added to the nutrient medium.
The main composition of the nutrient medium contained macro and micro salts according to Murasige and Skoog, double amounts of iron chelate; vitamins: ascorbic acid 1 mg L-1, thiamine 10 mg L-1, pyridoxine 5.5 mg L-1, nicotinic acid 4.5 mg L-1, paraaminobenzoic acid 5 mg L-1, mesoynosite (75 mg L-1); carbohydrates: sucrose (30 g L-1). At the stage of rhizogenesis, the concentration of macro sols was halved, and the sucrose concentration was reduced to 20 g L-1. The percentage of adapted basal plants in the adaptation of microclonal plants under ex vitro conditions averaged 70% for the cultivars.
Virus-free seedlings were obtained for the establishment of basic and basic nursery stock to produce certified planting material.
As a result of the conducted studies, viral infection of mother plants was studied by ELISA, herbaceous indicators (for sap-transmissible viruses) and PCR. Selected strawberry cultivars were introduced into the tissue culture for improvement and propagation, with the improvement of the technological scheme of clonal micro- propagation specifically for each cultivar at the stages of explant sterilization, introduction into tissue culture, primary regeneration of apexes, proliferation and rhizogenesis with genotype preservation.
We have developed a biotechnology of in vitro micropropagation of garden strawberries and obtaining planting material free from viral and mycoplasma infections.
Explants were cultured on agarized nutrient medium at the stage of tissue culture and regeneration without growth regulators, with ATP and proline amino acid 1 mg L-1 each in the nutrient medium; at the stage of proliferation, cytokinin 6 benzylaminopurine (6BAP) was added to the nutrient medium; at the stage of rhizogenesis, kinetin and IBA (indole butyric acid) were added to the nutrient medium.
The main composition of the nutrient medium contained macro and micro salts according to Murasige and Skoog, double amounts of iron chelate; vitamins: ascorbic acid 1 mg L-1, thiamine 10 mg L-1, pyridoxine 5.5 mg L-1, nicotinic acid 4.5 mg L-1, paraaminobenzoic acid 5 mg L-1, mesoynosite (75 mg L-1); carbohydrates: sucrose (30 g L-1). At the stage of rhizogenesis, the concentration of macro sols was halved, and the sucrose concentration was reduced to 20 g L-1. The percentage of adapted basal plants in the adaptation of microclonal plants under ex vitro conditions averaged 70% for the cultivars.
Virus-free seedlings were obtained for the establishment of basic and basic nursery stock to produce certified planting material.
Authors
A.K. Tashkenbayeva, M.Zh. Sarshayeva
Keywords
garden strawberry, nutrient medium, in vitro, clonal micropropagation, ex vitro, adaptation
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