Articles
Thidiazuron stimulates in vitro shoot regeneration from cotyledonary node explants in mango
Article number
1415_14
Pages
129 – 134
Language
English
Abstract
Mango vegetative propagation is slow and only allows the production of a relatively small amount of plant material when compared to other fruit tree crops.
High explant phenolic exudation and necrosis, media browning or slow in vitro growth makes mango a recalcitrant species.
Currently, no efficient methods for mango in vitro establishment, micropropagation, or shoot regeneration are available.
Previous studies in our group revealed the cotyledonary node (CN) in mango as a reactive explant.
CN consists of the embryo axis where cotyledons, main stem and root are inserted.
In this study, mature seeds of the polyembryonic cultivar ‘Ataulfo’ and the monoembryonic cultivar ‘Irwin’ were used.
Seeds were placed onto germination medium supplemented with 0, 1, 2 or 3 mg L‑1 of thidiazuron (TDZ). After 3 weeks of culture the percentage of CN showing adventitious shoot regeneration and the number of buds/shoots per regenerating explant were significantly higher (p<0.05) in the TDZ treatments compared with the treatment without TDZ. No statistically significant differences were found among the three TDZ concentrations for any of the parameters assessed.
For the two genotypes studied, regeneration rates reached approximately 90% when TDZ was applied.
Furthermore, TDZ treatments induced high-frequency of regeneration patterns, up to more than 6 buds/shoots per regenerating explant.
Taking into account mango recalcitrance, our results represent a significant improvement for in vitro plant regeneration/mass propagation of this species.
High explant phenolic exudation and necrosis, media browning or slow in vitro growth makes mango a recalcitrant species.
Currently, no efficient methods for mango in vitro establishment, micropropagation, or shoot regeneration are available.
Previous studies in our group revealed the cotyledonary node (CN) in mango as a reactive explant.
CN consists of the embryo axis where cotyledons, main stem and root are inserted.
In this study, mature seeds of the polyembryonic cultivar ‘Ataulfo’ and the monoembryonic cultivar ‘Irwin’ were used.
Seeds were placed onto germination medium supplemented with 0, 1, 2 or 3 mg L‑1 of thidiazuron (TDZ). After 3 weeks of culture the percentage of CN showing adventitious shoot regeneration and the number of buds/shoots per regenerating explant were significantly higher (p<0.05) in the TDZ treatments compared with the treatment without TDZ. No statistically significant differences were found among the three TDZ concentrations for any of the parameters assessed.
For the two genotypes studied, regeneration rates reached approximately 90% when TDZ was applied.
Furthermore, TDZ treatments induced high-frequency of regeneration patterns, up to more than 6 buds/shoots per regenerating explant.
Taking into account mango recalcitrance, our results represent a significant improvement for in vitro plant regeneration/mass propagation of this species.
Publication
Authors
E. Carmona-Martín, F. Conde, J.I. Hormaza, C. Petri
Keywords
Anacardiaceae, organogenesis, shoot multiplication, fruit crops, Mangifera, thidiazuron, cotyledonary node
Groups involved
Online Articles (38)