Articles
Isolation and expression analysis of glutathione S-transferase (GST)-like genes involved in anthocyanin accumulation in mango peel
Article number
1415_21
Pages
187 – 192
Language
English
Abstract
Recently, genes involved in anthocyanin biosynthesis in mango peel have been isolated.
Expression analysis of these genes during red coloration in ‘Irwin’ peel showed that the expression of MiCHS, MiANS, MiUFGT1, and MiUFGT3 is deeply involved in mango peel coloration and is regulated by the transcription factor MiMYB1. However, factors involved in anthocyanin transport and accumulation in mango peel have not been analyzed.
It has been reported that glutathione S-transferase (GST) is involved in anthocyanin transport and accumulation in some plants.
In this study, we isolated GST-like genes from the peel of ‘Irwin’ and investigated their relationship with anthocyanin accumulation.
We identified four GST-like genes from transcriptome data obtained from the ‘Irwin’ peel using local blast searches and named them MiGST1-4. In MiGST1, eight of the nine conserved amino acid residues that are present in known GST proteins involved in anthocyanin transport were conserved, but these residues were not conserved in MiGST2-4. Analysis of the expression of these genes in mango peel showed that MiGST1 was not expressed in fruit in which anthocyanin accumulation was suppressed by bagging (shading), but only in de-bagged fruit in which anthocyanin accumulation was observed.
On the other hand, the relationship between the expression of MiGST2-4 and anthocyanin accumulation was not clear.
These results suggest that MiGST1 is a GST protein responsible for anthocyanin transport in mango peel.
Expression analysis of these genes during red coloration in ‘Irwin’ peel showed that the expression of MiCHS, MiANS, MiUFGT1, and MiUFGT3 is deeply involved in mango peel coloration and is regulated by the transcription factor MiMYB1. However, factors involved in anthocyanin transport and accumulation in mango peel have not been analyzed.
It has been reported that glutathione S-transferase (GST) is involved in anthocyanin transport and accumulation in some plants.
In this study, we isolated GST-like genes from the peel of ‘Irwin’ and investigated their relationship with anthocyanin accumulation.
We identified four GST-like genes from transcriptome data obtained from the ‘Irwin’ peel using local blast searches and named them MiGST1-4. In MiGST1, eight of the nine conserved amino acid residues that are present in known GST proteins involved in anthocyanin transport were conserved, but these residues were not conserved in MiGST2-4. Analysis of the expression of these genes in mango peel showed that MiGST1 was not expressed in fruit in which anthocyanin accumulation was suppressed by bagging (shading), but only in de-bagged fruit in which anthocyanin accumulation was observed.
On the other hand, the relationship between the expression of MiGST2-4 and anthocyanin accumulation was not clear.
These results suggest that MiGST1 is a GST protein responsible for anthocyanin transport in mango peel.
Publication
Authors
S. Kanzaki, Y. Tanaka, A. Ichihi, K. Shimizu
Keywords
bagging, fruit coloration, Mangifera indica, MiMYB1
Groups involved
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