Articles
Confirming the identity of blueberry cultivars with a microsatellite marker-based fingerprinting set
Article number
1440_14
Pages
99 – 106
Language
English
Abstract
The United States Department of Agriculture (USDA) Agricultural Research Service (ARS) National Clonal Germplasm Repository (NCGR) in Corvallis, Oregon conserves a collection of more than 1,800 Vaccinium L. accessions representing 83 taxa from 34 countries.
Verifying the genotypic identity of blueberry cultivars preserved at the NCGR is key for efficient genebank management.
Previously, applying a 10-SSR fingerprinting set in blueberry identified four categories of plants: true-to-type (TTT) where morphology, SSR markers, and parentage analysis agreed; identity ok (IDOK) where a unique genotype was generated in multiple plants from different sources but parentage analysis was incomplete; identity question (IDQ) where allele composition did not match parentage and more testing is needed to confirm identity; and identity wrong (IDX) where incorrect identity is confirmed by parentage analysis and replacement where TTT is required.
The objectives of this study were to confirm the identity of cultivars in the IDOK (11) category, resolve the identity of cultivars in the IDQ (14) class, replace accessions in the IDX (6) category with TTT genotypes, and establish baseline fingerprints for the remaining cultivated blueberries (57) in the NCGR collection.
Plants for most accessions in the IDOK, IDQ, and IDX categories were obtained from up to seven sources including four nurseries and three breeders.
Genotyping 138 plants with the 10-SSR set identified 18 homonym and 13 synonym sets.
Pedigree analysis confirmed trueness-to type of 15 cultivars including two previously classified as IDOK category (‘Bluechip’ and ‘Berkeley’), three IDQ (‘Brightwell’, NC 1528, and ‘Toro’), and three IDX (‘Briteblue’, ‘Darrow’, and ‘Woodard’). This fingerprinting set and database are valuable to ensure clonal identity of the NCGR blueberry collection and will be used to identify unknown cultivars and verify the identity of existing and new accessions.
Verifying the genotypic identity of blueberry cultivars preserved at the NCGR is key for efficient genebank management.
Previously, applying a 10-SSR fingerprinting set in blueberry identified four categories of plants: true-to-type (TTT) where morphology, SSR markers, and parentage analysis agreed; identity ok (IDOK) where a unique genotype was generated in multiple plants from different sources but parentage analysis was incomplete; identity question (IDQ) where allele composition did not match parentage and more testing is needed to confirm identity; and identity wrong (IDX) where incorrect identity is confirmed by parentage analysis and replacement where TTT is required.
The objectives of this study were to confirm the identity of cultivars in the IDOK (11) category, resolve the identity of cultivars in the IDQ (14) class, replace accessions in the IDX (6) category with TTT genotypes, and establish baseline fingerprints for the remaining cultivated blueberries (57) in the NCGR collection.
Plants for most accessions in the IDOK, IDQ, and IDX categories were obtained from up to seven sources including four nurseries and three breeders.
Genotyping 138 plants with the 10-SSR set identified 18 homonym and 13 synonym sets.
Pedigree analysis confirmed trueness-to type of 15 cultivars including two previously classified as IDOK category (‘Bluechip’ and ‘Berkeley’), three IDQ (‘Brightwell’, NC 1528, and ‘Toro’), and three IDX (‘Briteblue’, ‘Darrow’, and ‘Woodard’). This fingerprinting set and database are valuable to ensure clonal identity of the NCGR blueberry collection and will be used to identify unknown cultivars and verify the identity of existing and new accessions.
Publication
Authors
O. Yalcin, R. King, A. Nyberg, J.M. Oliphant, N.V. Bassil
Keywords
SSRs, Vaccinium corymbosum, highbush blueberry, National Clonal Germplasm Repository, NCGR, blueberry collection, genotyping
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