Articles
Diversity of direct regeneration rate in highbush blueberry cultivars
Article number
1440_9
Pages
65 – 70
Language
English
Abstract
Plants possess a versatile and robust regeneration capability that can give rise to a whole plant from a single cell.
Plant regeneration is a prerequisite step required for applications of biotechnological technique to propagation and breeding, such as rapid proliferation of clonal plants, genetic transformation, and genome editing.
There are several ways for in vitro plant regeneration, with the most used being indirect regeneration (callus-mediated regeneration) and somatic embryogenesis.
In contrast, direct regeneration, characterized by shoot organogenesis without prominent callus formation, holds potential for expediting genetic transformation with minimizing somatic mutations associated with callus culture.
Plant regeneration is significantly affected by genotype and culture conditions such as phytohormones composition.
In this study we found that the ratio of cytokinin to auxin in the regeneration medium is a crucial factor for regeneration rate and auxin supplementation tended to promote callus formation and indirect regeneration but suppressed direct shoot regeneration.
We also found that ‘Blue Muffin’, ‘Brigitta’, and ‘Spartan’ exhibited high regeneration rate (>75%) while ‘Biloxi’, ‘Georgiagem’, ‘Gulfcoast’, ‘O’Neal’, and ‘Sharpblue’ exhibited low regeneration rate (<40%), suggesting that direct regeneration potential significantly differs across cultivars.
The reported and this study collectively suggest that genetic factors are relevant to direct regeneration capacity and further studies on the genetic factors controlling direct regeneration may be useful to establish high plant regeneration system in blueberry.
Plant regeneration is a prerequisite step required for applications of biotechnological technique to propagation and breeding, such as rapid proliferation of clonal plants, genetic transformation, and genome editing.
There are several ways for in vitro plant regeneration, with the most used being indirect regeneration (callus-mediated regeneration) and somatic embryogenesis.
In contrast, direct regeneration, characterized by shoot organogenesis without prominent callus formation, holds potential for expediting genetic transformation with minimizing somatic mutations associated with callus culture.
Plant regeneration is significantly affected by genotype and culture conditions such as phytohormones composition.
In this study we found that the ratio of cytokinin to auxin in the regeneration medium is a crucial factor for regeneration rate and auxin supplementation tended to promote callus formation and indirect regeneration but suppressed direct shoot regeneration.
We also found that ‘Blue Muffin’, ‘Brigitta’, and ‘Spartan’ exhibited high regeneration rate (>75%) while ‘Biloxi’, ‘Georgiagem’, ‘Gulfcoast’, ‘O’Neal’, and ‘Sharpblue’ exhibited low regeneration rate (<40%), suggesting that direct regeneration potential significantly differs across cultivars.
The reported and this study collectively suggest that genetic factors are relevant to direct regeneration capacity and further studies on the genetic factors controlling direct regeneration may be useful to establish high plant regeneration system in blueberry.
Publication
Authors
M. Omori, H. Yamane, R. Tao
Keywords
blueberry, direct regeneration, phytohormone, genotype
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