Articles
Development of reliable screening methods for bacterial blight in highbush blueberry
Article number
1440_24
Pages
171 – 176
Language
English
Abstract
Highbush blueberry production is impacted by several diseases.
Bacterial blight caused by Pseudomonas syringae is an important one.
Most of the highbush blueberry cultivars including new high-yielding cultivars are susceptible to this disease, therefore, breeding programs are required to prioritize developing disease resistant cultivars.
There are no reliable or effective methods available to screen highbush blueberry against bacterial blight.
We evaluated P. syringae inoculation methods on detached leaves in a moist chamber as well as on leaves of intact plants in controlled environments.
Detached leaf assay was effective in determining the pathogenicity of P. syringae rapidly.
All three inoculation methods on intact plants were effectively inducing bacterial blight symptoms on the foliage of the susceptible cultivar ‘Draper’. These methods are required to compare the different cultivars of highbush blueberry in the future to determine the discriminatory ability of these assays for levels of disease resistance.
Furthermore, field validation of screening methods are also required.
The development of reliable screening methods will help highbush blueberry breeding programs toward developing cultivars resistant to bacterial blight.
Bacterial blight caused by Pseudomonas syringae is an important one.
Most of the highbush blueberry cultivars including new high-yielding cultivars are susceptible to this disease, therefore, breeding programs are required to prioritize developing disease resistant cultivars.
There are no reliable or effective methods available to screen highbush blueberry against bacterial blight.
We evaluated P. syringae inoculation methods on detached leaves in a moist chamber as well as on leaves of intact plants in controlled environments.
Detached leaf assay was effective in determining the pathogenicity of P. syringae rapidly.
All three inoculation methods on intact plants were effectively inducing bacterial blight symptoms on the foliage of the susceptible cultivar ‘Draper’. These methods are required to compare the different cultivars of highbush blueberry in the future to determine the discriminatory ability of these assays for levels of disease resistance.
Furthermore, field validation of screening methods are also required.
The development of reliable screening methods will help highbush blueberry breeding programs toward developing cultivars resistant to bacterial blight.
Publication
Authors
R.R. Burlakoti, S.R. Latchman, A. Novinscak
Keywords
Pseudomonas syringae, inoculation, resistance, draper, field
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