Articles
Molecular identification of subpopulation Botrytis cinerea and susceptibility to fungicides isolates from blueberry (Vaccinium corymbosum) in Peru
Article number
1440_45
Pages
325 – 332
Language
English
Abstract
Peru is one of the main world producers of blueberries.
However, it presents phytosanitary challenges during the production process.
One of the most important diseases is gray mold caused by Botrytis spp., which causes losses of up to 30% of the berries in the postharvest stage.
Therefore, the main objective of this research was to identify and characterize eight Botrytis spp. isolates, from the main blueberry producing areas and evaluate their sensitivity to different active ingredients (a.i.). Collections were made from the regions of La Libertad, Lima, Ica, and Ancash.
To molecular identify the genes, the internal transcribed spacer region (its), glyceraldehyde-3-phosphate dehydrogenase (g3pdh), heat-shock protein 60 (hsp60), RNA polymerase II second largest subunit (rpb2) and multidrug resistance regulator 1 (mrr1) were used.
The morphological and cultural aspect of the studied isolates was characterized.
Additionally, the sensitivity to seven a.i. by inhibiting mycelial growth using YBA and MEA media and PDA medium.
As a result, the eight isolates belong to the Botrytis cinerea sensu stricto.
The frequency of subpopulation N was 75 and 25% to subpopulation S. Two colony types were observed on PDA, sclerotial (six isolates) and mycelial (two isolates). The isolates were susceptible to boscalid, fludioxonil, and fluopyram; however, resistance was shown to iprodione and thiophanate-methyl.
These results establish a baseline in understanding the behavior of the reaction to the different a.i. within the chemical control component in the IPM of the disease under Peruvian conditions
However, it presents phytosanitary challenges during the production process.
One of the most important diseases is gray mold caused by Botrytis spp., which causes losses of up to 30% of the berries in the postharvest stage.
Therefore, the main objective of this research was to identify and characterize eight Botrytis spp. isolates, from the main blueberry producing areas and evaluate their sensitivity to different active ingredients (a.i.). Collections were made from the regions of La Libertad, Lima, Ica, and Ancash.
To molecular identify the genes, the internal transcribed spacer region (its), glyceraldehyde-3-phosphate dehydrogenase (g3pdh), heat-shock protein 60 (hsp60), RNA polymerase II second largest subunit (rpb2) and multidrug resistance regulator 1 (mrr1) were used.
The morphological and cultural aspect of the studied isolates was characterized.
Additionally, the sensitivity to seven a.i. by inhibiting mycelial growth using YBA and MEA media and PDA medium.
As a result, the eight isolates belong to the Botrytis cinerea sensu stricto.
The frequency of subpopulation N was 75 and 25% to subpopulation S. Two colony types were observed on PDA, sclerotial (six isolates) and mycelial (two isolates). The isolates were susceptible to boscalid, fludioxonil, and fluopyram; however, resistance was shown to iprodione and thiophanate-methyl.
These results establish a baseline in understanding the behavior of the reaction to the different a.i. within the chemical control component in the IPM of the disease under Peruvian conditions
Publication
Authors
O. Alberca, C. Ureta, G. Ciprian, A. Casas, W. Apaza, L. Aragón, M. Huarhua
Keywords
Botrytis, media culture, mrr1, nuclear genes, fungicide resistant
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