Articles
SOMATIC EMBRYOGENESIS, ORGANOGENESIS AND PROLIFERATION IN VITRO FROM RUBUS EMBRYOS
Article number
183_11
Pages
91 – 98
Language
Abstract
Immature embryo rescue, embryogenesis, and organogenesis can expedite Rubus breeding through multiplication of desirable genotypes and initiation of variability.
Controlled interspecific crosses were made between ‘Black Satin’, ‘Hull Thornless’, ‘Cheyenne’, ‘Cherokee’, ‘Thornless Boysenberry’, ‘Marion’, and ‘Tayberry’. Ovules and embryos were aseptically excised at various stages of maturity, based on fruit color, and cultured.
Initiation media consisted of MS major and minor salts, Staba vitamins, 30 g/L sucrose, 200 to 500 mg/L casein hydrolysate, 0.1 mg/L 2,4-dichlorophenoxyacetic acid, and 0.1 or 1.0 mg/L benzylaminopurine.
Embryogenesis was observed from immature ovules and mature embryos after limited callus development, but shoot regeneration only occured from mature embryos.
Regenerated plantlets were isolated, rooted, screened for variation in the greenhouse, and are being evaluated in the field.
A low rate of within-seed variation is evident.
Controlled interspecific crosses were made between ‘Black Satin’, ‘Hull Thornless’, ‘Cheyenne’, ‘Cherokee’, ‘Thornless Boysenberry’, ‘Marion’, and ‘Tayberry’. Ovules and embryos were aseptically excised at various stages of maturity, based on fruit color, and cultured.
Initiation media consisted of MS major and minor salts, Staba vitamins, 30 g/L sucrose, 200 to 500 mg/L casein hydrolysate, 0.1 mg/L 2,4-dichlorophenoxyacetic acid, and 0.1 or 1.0 mg/L benzylaminopurine.
Embryogenesis was observed from immature ovules and mature embryos after limited callus development, but shoot regeneration only occured from mature embryos.
Regenerated plantlets were isolated, rooted, screened for variation in the greenhouse, and are being evaluated in the field.
A low rate of within-seed variation is evident.
Publication
Authors
Joseph A. Fiola, H. Swartz
Keywords
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