Articles
IN VITRO MUTAGENESIS IN ROSES
Article number
189_4
Pages
37 – 46
Language
Abstract
Within the scope of rose breeding programmes, in vitro mutagenesis – a combination of in vitro culture and mutation induction – provides the opportunity to increase variability of an economically important cultivar or to obtain information about the potential of spontaneously arising sports in a brand new variety.
A broad variability was established when applying X-ray doses between 25 Gy and 60 Gy to basal segments of in vitro derived microshoots of the cut rose cultivar ‘Ilseta’, followed by the subsequently repeated cutting off of axillary shoots from treated ‘mother’-explants.
The mutation spectrum, including tissue rearrangements etc. comprised 73 % flower mutants (size, colour, number of petals), 14 % with a changed growth-(archi-)type and 13 % with modified leaves.
The mutant rate – the percentage of plants carrying mutations in relation to the total number of inspected regenerated M1-plants – rose with increasing irradiation dose. ‘Ilseta’ belongs to a group of cultivars with relatively low radiosensitivity.
A great number of flower mutants was induced by the highest mutagenic treatment.
Only a period of about 9 months from X-ray application of microshoots up to fully developed blooming plants was required to select mutants ready for grafting onto rootstocks.
A broad variability was established when applying X-ray doses between 25 Gy and 60 Gy to basal segments of in vitro derived microshoots of the cut rose cultivar ‘Ilseta’, followed by the subsequently repeated cutting off of axillary shoots from treated ‘mother’-explants.
The mutation spectrum, including tissue rearrangements etc. comprised 73 % flower mutants (size, colour, number of petals), 14 % with a changed growth-(archi-)type and 13 % with modified leaves.
The mutant rate – the percentage of plants carrying mutations in relation to the total number of inspected regenerated M1-plants – rose with increasing irradiation dose. ‘Ilseta’ belongs to a group of cultivars with relatively low radiosensitivity.
A great number of flower mutants was induced by the highest mutagenic treatment.
Only a period of about 9 months from X-ray application of microshoots up to fully developed blooming plants was required to select mutants ready for grafting onto rootstocks.
Authors
F. Walther, A. Sauer
Keywords
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