Articles
STENTING OF ROSES, STARCH DEPLETION AND ACCUMULATION DURING THE EARLY DEVELOPMENT
Article number
189_6
Pages
51 – 60
Language
Abstract
Stenting of roses is a propagation technique, based on simultaneously cutting and grafting (Van de Pol et al., 1982). In the present study whip grafting was introduced with stock and scion held together with a clothes-peg.
Microscopical investigation of the stem above and below the graft union showed a marked increase of the starch content of the scion, particularly during the first week after grafting.
Starch in the rootstock shows a sharp decrease, directly after grafting, reaches level 0 on day 3 and subsequently increases until after 12–15 days it approaches the level of the scion.
At day 12 from grafting 100% of the plants had formed a xylem vessel connection as shown by colouring with acid fuchsine, and 15 days from grafting 75% were rooted.
The increase of percentages of rootstocks with starch from day 3 onwards preceeds the increase of percentages of stentlings with xylem vessel connection by about 2 days.
The CO2 concentration in the propagation bench decreased from more than 1500 ppm during the night to less than 100 ppm during the day.
This change in CO2 concentration and the pattern of the starch accumulation in the stem prove the activity of the scion leaf.
This is a necessity for the development of roots and shoots on the young stentling.
Microscopical investigation of the stem above and below the graft union showed a marked increase of the starch content of the scion, particularly during the first week after grafting.
Starch in the rootstock shows a sharp decrease, directly after grafting, reaches level 0 on day 3 and subsequently increases until after 12–15 days it approaches the level of the scion.
At day 12 from grafting 100% of the plants had formed a xylem vessel connection as shown by colouring with acid fuchsine, and 15 days from grafting 75% were rooted.
The increase of percentages of rootstocks with starch from day 3 onwards preceeds the increase of percentages of stentlings with xylem vessel connection by about 2 days.
The CO2 concentration in the propagation bench decreased from more than 1500 ppm during the night to less than 100 ppm during the day.
This change in CO2 concentration and the pattern of the starch accumulation in the stem prove the activity of the scion leaf.
This is a necessity for the development of roots and shoots on the young stentling.
Authors
P.A. van de Pol, M.H.A.J. Joosten, H. Keizer
Keywords
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