Articles
VIRUSES CAPABLE OF CAUSING A SCORCH DISEASE OF HIGHBUSH BLUEBERRY.
Article number
241_50
Pages
295 – 300
Language
Abstract
A carlavirus, designated blueberry scorch carlavirus (BBScV) has been shown recently to cause a scorch disease of highbush blueberry.
During a survey of blueberry fields in British Columbia, Washington, and Oregon BBScV was found to be restricted to the Puyallup Valley in Washington.
However, there were scorched blueberry bushes in all areas surveyed that did not react with our BBScV antiserum in double antibody sandwich ELISA. Purification from these bushes using the BBScV protocol was unsuccessful, suggesting that another causal agent induced scorch symptoms.
Flowers from some of these bushes in one field were used as inoculum for mechanical transmission to Nicotiana clevelandii. Purification from the Nicotiana plants yielded spherical particles when observed with the electron microscope.
A host range was conducted with this isolate and only three species of Nicotiana produced symptoms.
BBScV has not been mechanically transmitted to any host from the flowers or leaves.
Thin sections of leaves taken from other fields with BBScV-like symptoms were examined with the electron microscope and this revealed long, flexous, rod-shaped, virus-like particles.
This cytopathology is distinct from both BBScV particles or the spherical virus purified from Nicotiana.
During a survey of blueberry fields in British Columbia, Washington, and Oregon BBScV was found to be restricted to the Puyallup Valley in Washington.
However, there were scorched blueberry bushes in all areas surveyed that did not react with our BBScV antiserum in double antibody sandwich ELISA. Purification from these bushes using the BBScV protocol was unsuccessful, suggesting that another causal agent induced scorch symptoms.
Flowers from some of these bushes in one field were used as inoculum for mechanical transmission to Nicotiana clevelandii. Purification from the Nicotiana plants yielded spherical particles when observed with the electron microscope.
A host range was conducted with this isolate and only three species of Nicotiana produced symptoms.
BBScV has not been mechanically transmitted to any host from the flowers or leaves.
Thin sections of leaves taken from other fields with BBScV-like symptoms were examined with the electron microscope and this revealed long, flexous, rod-shaped, virus-like particles.
This cytopathology is distinct from both BBScV particles or the spherical virus purified from Nicotiana.
Publication
Authors
S.G. MacDonald, R.R. Martin, P.R. Bristow
Keywords
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