Articles
METHODOLOGY OF IN VITRO GAMMA RAYS IRRADIATIONS FROM LONICERA SPECIES; MUTANT DESCRIPTION AND BIOCHEMICAL CHARACTERIZATION.
Article number
320_16
Pages
119 – 126
Language
Abstract
Microcuttings from five species of honeysuckle were irradiated in Petri dishes at the time of subculturing.
The criterion of bud survival was defined as the percentage of buds providing shoots longer than 10 mm, after four months of post-irradiation culture.
Cultivar survival was investigated after irradiation with a range of gamma rays from 10 to 60 Gy.
A methodology of mutants isolation was developed to increase the chance for a mutation induced in one cell to give rise to a mutated sector in the regenerated plant.
Among 200 regenerated plants from irradiation tests on Lonicera nitida ‘Maïgrün’, nine phenotypically obvious mutants were observed.
They differ in leaf morphology, growth habit and vigour, and for chlorophyllian deficiencies.
No difference between the control and the mutants was found in peroxidase, malate deshydrogenase (MDH) and glutamate oxaloacetate tranferase (GOT) zymograms.
Only one very slender mutant has been characterized by the lack of a 52 kD band on the banding pattern of denaturated soluble proteins.
The criterion of bud survival was defined as the percentage of buds providing shoots longer than 10 mm, after four months of post-irradiation culture.
Cultivar survival was investigated after irradiation with a range of gamma rays from 10 to 60 Gy.
A methodology of mutants isolation was developed to increase the chance for a mutation induced in one cell to give rise to a mutated sector in the regenerated plant.
Among 200 regenerated plants from irradiation tests on Lonicera nitida ‘Maïgrün’, nine phenotypically obvious mutants were observed.
They differ in leaf morphology, growth habit and vigour, and for chlorophyllian deficiencies.
No difference between the control and the mutants was found in peroxidase, malate deshydrogenase (MDH) and glutamate oxaloacetate tranferase (GOT) zymograms.
Only one very slender mutant has been characterized by the lack of a 52 kD band on the banding pattern of denaturated soluble proteins.
Authors
J. Cambecèdes, M. Duron, L. Decourtye, R. Jalouzot
Keywords
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