LOW TEMPERATURE STORAGE FOR PRODUCTION MANAGEMENT OF IN-VITRO PLANTS: EFFECTS OF AIR TEMPERATURE AND LIGHT INTENSITY ON PRESERVATION OF PLANTLET DRY WEIGHT AND QUALITY DURING STORAGE.

Broccoli (Brassica oleracea L. cv.
Ryokurei) seedlings were germinated aseptically and cultured photoautotrophically in vitro for 3 weeks at 23°C air temperature and 160 μmol· m^-2· s^-1 photosynthetic photon flux density (PPFD), then stored at 5°, 10°, or 15°C under continuous illumination at 2 or 5 μmol· m^-2· s^-1 PPFD. Dry weight, photosynthetic and regrowth ability of plantlets were best preserved at 5°C under 2 μmol· m^-2· s^-1 PPFD. At 10° and 15°C, photosynthetic and regrowth ability of plantlets after storage were higher under 5 than 2 μmol· m^-2· s^-1 PPFD, and decreased as air temperature or duration of storage increased.
Under 5 μmol· m^-2· s^-1 PPFD, plantlets elongated at 10° and 15°C during storage.
Dry weight of plantlets at 10°C under 5 μmol· m^-2· s^-1 PPFD increased during storage.
These results suggest that proper combinations of air temperature and PPFD during storage would contribute to minimizing increase/decrease in dry weight and preserving photosynthetic and regrowth ability of plantlets.

Environmental Effects and their Control in Plant Tissue Culture

C. Kubota, G. Niu, T. Kozai

chlorophyll fluorescence, light compensation point, micropropagation

https://doi.org/10.17660/ActaHortic.1995.393.11