EFFECTS OF RED AND FAR-RED LIGHT ON THE GROWTH AND MORPHOLOGY OF POTATO PLANTLETS IN VITRO: USING LIGHT EMITTING DIODE AS A LIGHT SOURCE FOR MICROPROPAGATION.

Red (peak wavelength: 660 nm) light emitting diodes (LEDs), far-red (peak wavelength: 730 nm) LEDs, white fluorescent lamps and blue fluorescent lamps were used as light sources.
Photosynthetic photon flux density (PPFD) on the empty culture shelf was adjusted at 100 μmol m^-2 s^-1 in all the treatments.
Red photon flux density (R) and far-red photon flux density (FR) were modified, respectively, in ranges between 10 and 100 μmol m^-2 s^-1, and between 2 and 50 μmol m^-2 s^-1. Blue photon flux density was adjusted at 10 μmol m^-2 s^-1 in all the treatments except for the treatment with R of 100 μmol m^-2 s^-1. Potato plantlets were cultured on Murashige and Skoog sugar-free agar medium at air temperatures of 24/20°C (photoperiod/dark period), and a photoperiod of 16 h d^-1. Shoot length increased with increasing FR/PPFD ratio when R/PPFD ratio was 0.1–0.5. Shoot length was greatest, and dry weight and leaf area were smallest with R/PPFD ratio of 1.0. R/PPFD ratio and FR/PPFD ratio affected the growth, morphology and photosynthesis of the plantlets.
Possible application of LEDs to control the growth, morphology and photosynthesis of the plantlets in vitro is discussed.

Environmental Effects and their Control in Plant Tissue Culture

Y. Miyashita, Y. Kitaya, T. Kozai, T. Kimura

far-red, light emitting diode, plant tissue culture, potato, red

https://doi.org/10.17660/ActaHortic.1995.393.22