Articles
THE ROLE OF THE GAS PHASE IN IN VITRO CULTURE OF PRUNUS AVIUM SHOOTS
Article number
393_27
Pages
227 – 230
Language
Abstract
Shoots of Prunus avium grown in vitro showed a clear morphological and physiological response to different CO2 and O2 concentrations: 0%CO2-21%O2, 0.09%CO2-8%O2, 0.034%CO2-21%O2. After 30 days shoots grown at 8% O2 and 0.09% CO2 showed higher fresh weight, higher % dry matter, higher lignification, lower chlorophyll content with anthocianins as compared with shoots grown at 0% and 0.034%CO2 respectively.
In plantlets grown in these conditions the ratio between chlorophyll a and b also changed as compared with shoots grown at 0.034%CO2-21%O2. Furthermore shoots grown at 0% CO2 were pale green colour during the first 15 days of culture followed by a remarkable regreening and the growth rate was always lower than in cultures enriched with CO2.
In plantlets grown in these conditions the ratio between chlorophyll a and b also changed as compared with shoots grown at 0.034%CO2-21%O2. Furthermore shoots grown at 0% CO2 were pale green colour during the first 15 days of culture followed by a remarkable regreening and the growth rate was always lower than in cultures enriched with CO2.
Authors
B. Righetti, T.A. Thorpe, D.M. Reid, A. Rotondi
Keywords
carbon dioxyde, micropropagation, oxygen, Prunus avium
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