Articles
THE CONTROL OF BIOREACTOR ENVIRONMENT FOR PLANT PROPAGATION IN LIQUID CULTURE
Article number
393_3
Pages
25 – 38
Language
Abstract
The establishment of efficient automated micropropagation to overcome some of the present labor input limitation requires, among other technologies, the development of scaled-up liquid bioreactor cultures.
Bioreactors are used in micropropagation for both the embryogenic and organogenic regeneration pathways.
However, regulatory systems for the control of the internal environment and for defining the requirements for quality plant regeneration in bioreactors are still limited to a few crop species.
Various factors in the vessel determine biomass production and propagule quality.
The type of bioreactor and the method of aeration and circulation have a major effect on the physical and chemical environment.
Effects of agitation, shearing forces, gaseous environment, pH, light, temperature, medium components, rheology and osmoticum and the level of growth promoting and growth inhibiting regulators will be discussed in relation to plant growth and morphogenesis in liquid cultures.
Bioreactors are used in micropropagation for both the embryogenic and organogenic regeneration pathways.
However, regulatory systems for the control of the internal environment and for defining the requirements for quality plant regeneration in bioreactors are still limited to a few crop species.
Various factors in the vessel determine biomass production and propagule quality.
The type of bioreactor and the method of aeration and circulation have a major effect on the physical and chemical environment.
Effects of agitation, shearing forces, gaseous environment, pH, light, temperature, medium components, rheology and osmoticum and the level of growth promoting and growth inhibiting regulators will be discussed in relation to plant growth and morphogenesis in liquid cultures.
Authors
M. Ziv
Keywords
bioreactor, aeration, agitation, automation, environmental control, liquid medium, micropropagation
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