DEVELOPMENT OF PHOTOAUTOTROPHIC PLANT TISSUE CULTURE SYSTEM USING CO₂ FROM SHIITAKE MUSHROOM

Growth characteristics were compared for plantlets cultured photoautotrophically with and without CO₂ produced by respiration of mycelia of shiitake mushroom (Lentinus edodes Sing.). Plantlets were cultured on a sugar-free medium in plant culture vessels at photosynthetic photon flux density of 90 μmol m^-2 s^-1 and air temperature of 23–25°C. In the treatment with CO₂ from mushroom, a plant culture chamber for plant culture vessels was combined tightly with a mushroom culture chamber.
CO₂ concentration in the plant culture chamber was controlled at about 1000 μmol mo1^-1. CO₂ concentrations inside the plant culture vessels during the photoperiod were 300–600 μmol mol^-1 with CO₂ from mushroom and 100 μmol mol^-1 without CO₂ from mushroom.
Dry weights of mint (Mentha rotundifolia L. Huds. cv.
Apple mint) and potato (Solanum tuberosum L. cv.
Benimaru) plantlets cultured with CO₂ from mushroom for 17 days were 1.5 and 1.3 times, respectively, those without CO₂ from mushroom.

Environmental Effects and their Control in Plant Tissue Culture

Y. Kitaya, K. Sakami, T. Kozai

CO₂, mushroom, photoautotrophic culture, photosynthesis, plant tissue culture, respiration

https://doi.org/10.17660/ActaHortic.1995.393.23