Articles
CHIMERA SYNTHESIS AND PROPAGATION SYSTEMS BY IN VIVO AND IN VITRO GRAFT METHODS IN BRASSICA
Article number
407_27
Pages
227 – 232
Language
Abstract
In vivo and in vitro chimera synthesis methods were established for Brassica crops.
As in vivo grafting method was not efficient to obtain sufficient number of chimeras, approach-grafted seedling culture (AGSC) method was finally adopted in synthesizing interspecific Brassica chimeras.
Vegetative progenies are stably obtained by the method.
Interspecific chimeras between B. campestris-B. oleracea produced mericlinal types, while, interspecific chimeras between B. napus-B. oleracea produced periclinal types.
The mixed type of chimera is best for maintaining the various types of chimeras in vegetative propagation systems from chimeral plants.
As in vivo grafting method was not efficient to obtain sufficient number of chimeras, approach-grafted seedling culture (AGSC) method was finally adopted in synthesizing interspecific Brassica chimeras.
Vegetative progenies are stably obtained by the method.
Interspecific chimeras between B. campestris-B. oleracea produced mericlinal types, while, interspecific chimeras between B. napus-B. oleracea produced periclinal types.
The mixed type of chimera is best for maintaining the various types of chimeras in vegetative propagation systems from chimeral plants.
Authors
S. Oguni, Y. Hirata, T. Kan, M. Kita, H. Horiuchi, T. Noguchi
Keywords
Grafting, Tissue culture, Red cabbage, Komatsuna, Rape
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