Articles
MASS PROPAGATION OF SYZYGIUM CUMINII FROM SELECTED ELITE TREES
Article number
429_62
Pages
489 – 496
Language
Abstract
Multiple shoots were obtained from nodal explants of 10-year-old elite trees of Syzygium cuminii on Murashige and skoog’s medium supplemented with 2.5 mgl-1 kinetin (Kn). Explants taken from in vitro proliferated shoots produced further multiple shoots when cultured on the same basal medium containing 2.5 mgl-1 Kn.
Repeated subculture resulted in rapid shoot multiplication at an average rate of 10 shoots per subculture.
Addition of 100 mgl-1 urea in the medium enhanced the number of shoots up to 60 per culture and incorporation of 100 mgl-1 casein hydrolysate (CH) and 15% (v/v) coconut milk (CM) to the medium increased the length of shoots.
In vitro raised shoots rooted on half strength MS medium containing 0.5 mgl-1 each of indole -3- butyricacid (IBA),
-naphthaleneaceticacid (NAA) and indole-3-aceticacid (IAA). After transplanting in the field, 75% plantlets survived with uniform growth.
Repeated subculture resulted in rapid shoot multiplication at an average rate of 10 shoots per subculture.
Addition of 100 mgl-1 urea in the medium enhanced the number of shoots up to 60 per culture and incorporation of 100 mgl-1 casein hydrolysate (CH) and 15% (v/v) coconut milk (CM) to the medium increased the length of shoots.
In vitro raised shoots rooted on half strength MS medium containing 0.5 mgl-1 each of indole -3- butyricacid (IBA),
-naphthaleneaceticacid (NAA) and indole-3-aceticacid (IAA). After transplanting in the field, 75% plantlets survived with uniform growth.
Authors
P.K. Roy, M. Rahman, S.K. Roy
Keywords
Clonal propagation, In vitro culture, Syzygium cuminii
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