Articles
IN VITRO PROPAGATION AND ESTABLISHMENT OF A NEW CULTIVAR OF JACKFRUIT (ARTOCARPUS HETEROPHYLLUS) BEARING FRUITS TWICE YEARLY
Article number
429_63
Pages
497 – 502
Language
Abstract
An in vitro culture method for clonal propagation of a horticulturally high yielding strain of jackfruit (Artocarpus heterophyllus) bearing fruits twice yearly has been achieved.
A few trees of jackfruit bearing fruits twice a year were selected and in vitro culture was employed for their clonal propagation, using shoot buds as explants.
Results showed that addition of 2.5 mg l-1 6-benzyladenine (BA) and 0.5 mgl-1
-naphthalene acetic acid (NAA) to MS nutrient medium induced maximum number of shoot buds.
These shoots continued to proliferate through several subcultures with an average of 10 shoots per subculture.
When the concentrations of BA and NAA were lowered to 1.25 mg l-1 and 0.25 mg l-1, respectively and 15% (v/v) coconut milk was added to the medium, the number of shoots increased up to 40. For rooting, well developed shoots were excised and implanted individually on rooting medium containing half strength MS salts supplemented with 1.0 mg l-1 each of NAA and indolebutyric acid (IBA). Young rooted plantlets were transplanted directly from the culture tube to earthen pots containing sterile sand, soil and humus (1:2:1) and covered by transparent plastic bags.
After acclimatization the potted plants were transplanted in the open field where 75% plants survived.
A few trees of jackfruit bearing fruits twice a year were selected and in vitro culture was employed for their clonal propagation, using shoot buds as explants.
Results showed that addition of 2.5 mg l-1 6-benzyladenine (BA) and 0.5 mgl-1
-naphthalene acetic acid (NAA) to MS nutrient medium induced maximum number of shoot buds.These shoots continued to proliferate through several subcultures with an average of 10 shoots per subculture.
When the concentrations of BA and NAA were lowered to 1.25 mg l-1 and 0.25 mg l-1, respectively and 15% (v/v) coconut milk was added to the medium, the number of shoots increased up to 40. For rooting, well developed shoots were excised and implanted individually on rooting medium containing half strength MS salts supplemented with 1.0 mg l-1 each of NAA and indolebutyric acid (IBA). Young rooted plantlets were transplanted directly from the culture tube to earthen pots containing sterile sand, soil and humus (1:2:1) and covered by transparent plastic bags.
After acclimatization the potted plants were transplanted in the open field where 75% plants survived.
Authors
S.K. Roy, P.K. Roy
Keywords
Fruit plant, Clonal propagation, Shoot culture, Jack fruit, Artocarpus heterophyllus
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