Articles
REGENERATION OF PLANTLETS AND BULBLETS FROM EXPLANTS AND CALLUS OF ALLIUM AFLATUNENSE CULTIVARS AND SELECTION FROM INDIGENOUS ISRAELI ALLIUM AMPELOPRASUM
Article number
430_50
Pages
325 – 330
Language
Abstract
Allium aflatunense is a bulbous plant, grown in gardens and also as cut flowers.
A. aflatunense cultivars are propagated asexully from bulbs or bulblets.
The two main constraints on growing this plant are a low multiplication rate and the high cost of bulb production.
Tissue culture technology enables large scale propagation of plantlets and bulblets.
Protocols have been developed for initiating callus and regenerating whole plantlets and bulblets from explants derived from different organs of cvs. ‘Lucy Ball’; ‘Gladiator’ and ‘Purple Sensation’. Within a short period of time, hundreds of plantlets and bulblets were obtained from a single bulb.
Direct organogenesis of shoots or bulblets was obtained on Murashige and Skoog (MS) media containing NAA and BA. Plantlet regeneration from embryogenic like callus was obtained with different combinations of IAA and Kinetin.
Explants derived from young inflorescences gave the highest regeneration rate.
For further development small bulblets were subcultured onto media with a high sucrose levels, and were incubated at low temperature.
Shoot proliferation in selections made from native populations of A. ampeloprasum was induced from shoot tips cultured on MS medium containing a high ratio of Kinetin to IAA and from young florets cultured on MS media with NAA and BA.
A. aflatunense cultivars are propagated asexully from bulbs or bulblets.
The two main constraints on growing this plant are a low multiplication rate and the high cost of bulb production.
Tissue culture technology enables large scale propagation of plantlets and bulblets.
Protocols have been developed for initiating callus and regenerating whole plantlets and bulblets from explants derived from different organs of cvs. ‘Lucy Ball’; ‘Gladiator’ and ‘Purple Sensation’. Within a short period of time, hundreds of plantlets and bulblets were obtained from a single bulb.
Direct organogenesis of shoots or bulblets was obtained on Murashige and Skoog (MS) media containing NAA and BA. Plantlet regeneration from embryogenic like callus was obtained with different combinations of IAA and Kinetin.
Explants derived from young inflorescences gave the highest regeneration rate.
For further development small bulblets were subcultured onto media with a high sucrose levels, and were incubated at low temperature.
Shoot proliferation in selections made from native populations of A. ampeloprasum was induced from shoot tips cultured on MS medium containing a high ratio of Kinetin to IAA and from young florets cultured on MS media with NAA and BA.
Publication
Authors
D. Evenor, A. Levi-Nissim, L. Afgin, H. Lilien-Kipnis, A.A. Watad
Keywords
micropropagation, inflorescence, leaf bases, basal plate, shoot tips
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